黑果腺肋花楸黄酮提取物修复小鼠急性酒精肝损伤的研究  

Studies of the Effects of Flavone Extracts from Black Chokeberry on Acute Alcoholic Liver Injury in Mice

作　　者：郝思奥 李艳[1,2] 贺艳楠 吴楠 HAO Si-ao;LI Yan;HE Yan-nan;WU Nan(College of Science and Biology,Hebei University of Science and Technology,Shijiazhuang 050018,Hebei,China;R&D Center for Fermentation Engineering of Hebei Province,Shijiazhuang 050018,Hebei,China)

机构地区：[1]河北科技大学食品与生物学院,河北石家庄050018 [2]河北省发酵工程技术研究中心,河北石家庄050018

出　　处：《食品研究与开发》2021年第14期30-35,共6页Food Research and Development

基　　金：河北省重点研发计划项目(18227131D、19227128D)。

摘　　要：研究黑果腺肋花楸黄酮提取物对昆明小鼠急性酒精肝损伤的修复作用。以黑果腺肋花楸为原料,提取黄酮类化合物,分别以正常组为空白、模型组和药物组为对照,黄酮提取物高低剂量组饲喂黑果腺肋花楸黄酮提取物5周后,测定小鼠血清与肝组织谷草转氨酶(aspartate aminotransferase,AST)、谷丙转氨酶(alanine aminotransferase,ALT)、谷胱甘肽过氧化物酶(glutathione peroxidase,GSH-Px)活性、丙二醛(malondialdehyde,MDA)含量和总抗氧化能力(total antioxidant capacity,T-AOC)。模型组与正常组相比,肝脏及血清中AST、ALT活性升高,GSH-Px活性下降,MDA含量增加,T-AOC下降。低剂量组与模型组相比,肝脏及血清AST、ALT活性下降,GSH-Px活性升高,MDA含量降低,T-AOC升高;与正常组相比,肝脏及血清AST活性下降,GSH-Px活性提高,MDA含量降低,T-AOC升高;与高剂量组相比,肝脏及血清AST、ALT活性下降,MDA含量降低,T-AOC增加。黑果腺肋花楸黄酮提取物对小鼠急性酒精肝损伤有良好的修复效果,低剂量组效果更加明显。The reparative effects of flavonoid extracts from black chokeberry on acute alcoholic liver injury in Kunming mice were studied.Flavonoids were extracted from black chokeberry and fed to groups of alcoholic liver injury induced Kunming mice at high or low doses,with the normal group as blank and the model group as control.After five weeks of feeding,aspertate aminotransferase(AST),alanine aminotransferase(ALT),and glutathione peroxidase(GSH-Px)activity,and malondialdehyde(MDA)content and total antioxidant capacity(T-AOC)were assessed in serum and liver tissue from all mice.AST and ALT activity,GSH-Px activity,MDA content,and T-AOC activity in liver and serum of the injury model group were increased compared with that of the uninjured control group.Liver and serum AST and ALT activities were decreased,GSH-Px activity was increased,MDA content was decreased,and T-AOC was increased in the low-dose group relative to the injury model group.Compared with the uninjured control group,liver and serum AST activity were decreased,GSHPx activity was increased,MDA content was decreased,and T-AOC was increased.Compared to the high dose group,liver and serum AST and ALT activities were decreased,MDA content was decreased,and T-AOC was increased.Flavonoid extract from black chokeberry displayed a potent preventive effect on acute alcoholic liver injury in mice,with the more pronounced effect occurring in the low-dose group.

关 键 词：黑果腺肋花楸 抗氧化性 昆明小鼠 保肝功效 预防肝损伤 

分 类 号：R285.5[医药卫生—中药学]