双水相体系分离纯化紫甘薯花色苷  

Separation and purification of purple sweet potato anthocyanins by aqueous two-phase system

作　　者：方舟 张军兵 罗忠国 熊勇 罗舜菁 刘成梅 Fang Zhou;Zhang Junbing;Luo Zhongguo;Xiong Yong;Luo Shunjing;Liu Chengmei(School of Food Science,Nanchang University,Nanchang 330000;Jiangxi Danxia Biological Co.,Ltd.,Yingtan 335000)

机构地区：[1]南昌大学食品学院,南昌330000 [2]江西丹霞生物股份有限公司,鹰潭335000

出　　处：《粮食与食品工业》2021年第4期29-33,共5页Cereal & Food Industry

摘　　要：本研究采用磷酸二氢钠/乙醇双水相体系对紫甘薯花色苷进行分离纯化,通过单因素实验探究了磷酸二氢钠浓度、乙醇浓度、浓缩液添加量对糖残留率和花色苷回收率的影响,并通过正交实验优化其工艺条件。结果表明,以花色苷回收率为考察指标,三个因素的影响大小依次为浓缩液添加量、磷酸二氢钠浓度、乙醇浓度,最优工艺为磷酸二氢钠浓度为20%,乙醇浓度为25%,浓缩液添加量为0.6 mL,此时花色苷回收率为99.63%,糖残留率为55.78%;以糖残留率为考察指标,三个因素的影响大小依次为磷酸二氢钠浓缩,乙醇浓度,浓缩液添加量,最优工艺为磷酸二氢钠浓度为22%,乙醇浓度为30%,浓缩液添加量为0.6 mL,此时花色苷回收率为99.31%,糖残留率为42.51%。通过单因素实验和正交实验优化的工艺条件合理可靠,为紫甘薯色素的产业化和其开发利用提供理论依据。In this study,the purple sweet potato anthocyanins were separated and purified by a two-phase system of sodium dihydrogen phosphate/ethanol.Single factor experiments were used to explore the effects of sodium dihydrogen phosphate concentration,ethanol concentration,and concentrated solution addition on the sugar residue rate and anthocyanin recovery rate.And optimized its process conditions through orthogonal experiments.The results show that taking the anthocyanin recovery rate as the inspection index,the influence of the three factors is the amount of concentrated solution added,the concentration of sodium dihydrogen phosphate,and the concentration of ethanol.The optimal process is that the concentration of sodium dihydrogen phosphate is 20%,and the concentration of ethanol is 25%,and the added amount of concentrate is 0.6mL.At this time,the anthocyanin recovery rate is 99.63%,and the sugar residue rate is 55.78%;the sugar residue rate is used as the inspection index,and the influence of the three factors is the concentration of sodium dihydrogen phosphate,the concentration of ethanol and the amount of concentrate added.The optimal process is that the concentration of sodium dihydrogen phosphate is 22%,the concentration of ethanol is 30%,and the amount of concentrate added is 0.6 mL.At this time,the anthocyanin recovery rate is 99.31%,and the sugar residue rate is 42.51%.The process conditions optimized by single factor experiment and orthogonal experiment are reasonable and reliable,and provide a theoretical basis for the industrialization of purple sweet potato pigment and its development and utilization.

关 键 词：紫甘薯 双水相体系 糖残留率 花色苷回收率 

分 类 号：TS201[轻工技术与工程—食品科学]