VEGF与Survivin反义寡核苷酸对裸鼠荷舌癌肿瘤的作用  

Inhibitory effect of VEGF and Survivin antisense phosphorothioate oligodeoxynucleotides on the growth of human tongue cancer xenografts in nude mice

作　　者：李腾宇 于肖鹏[1] 李晓光[1] 王延秀[2] 李俊福 陈岱韻 孙振 Li Tengyu;Yu Xiaopeng;Li Xiaoguang;Wang Yanxiu;Li Junfu;Chen Daiyun;Sun Zhen(Dept.of Stomatology,Taian City Central Hospital,Taian 271000,China;Dept.of Pain,Taian City Central Hospital,Taian 271000,China;Dept.of Stomatology,Second Affiliated Hospital of Shandong First Medical University,Taian 27100,China)

机构地区：[1]泰安市中心医院口腔科,山东泰安271000 [2]泰安市中心医院疼痛科,山东泰安271000 [3]山东第一医科大学第二附属医院口腔科,山东泰安271000

出　　处：《山东第一医科大学（山东省医学科学院）学报》2021年第6期401-405,共5页Journal of Shandong First Medical University ＆ Shandong Academy of Medical Sciences

基　　金：山东省科学技术发展计划资助项目(2011GGH21822);泰安市科技局资助项目(2016NS1092)。

摘　　要：目的︰研究血管内皮细胞生长因子反义寡核苷酸(VECF-ASODN)和生存素(Survivinm)反义寡核苷酸(Survivin-ASODN)联合转染对裸鼠体内Tca8113舌癌细胞的作用。方法﹐25只雌性裸鼠分为5组,每组5只,建立移植瘤模型,并注入相应的试剂。①联合ASODN组:每次在裸鼠瘤体及瘤周注入Survivin-ASODN,VECF-ASODN各66μg,注射液体积为200μl,内含LipofectamineTM 2000和无血清1640液。②VECF-ASODN组;同上述方法注射VECF-ASODN 66μg。③Survivin-ASODN组:同法注射Survivin-ASODN 66μg。④脂质体组:同法注射等量脂质体200μl。⑤生理盐水(NS)组:注射等量NS200μl。3天注射1次,共7次。实验结束2天后,全麻下处死裸鼠,称取肿瘤重量并测量大小,肿瘤制作石蜡切片,免疫组织化学方法测VECF,Survivin,PCNA,CD34表达水平,计算肿瘤微血管密度(microvessel density,MVD)和肿瘤增殖细胞核抗原标记指数(PCNA Iabel index,PLI)。结果﹑联合ASODN组裸鼠移植瘤的重量、体积、VECF和Survivin蛋白,PCNA、CD34表达水平明显降低。结论VEGF-ASODN和Survivin-ASODN联合转染Tca8113舌癌细胞,明显抑制VECF和Survivin表达水平,并且抑制癌细胞生长和增殖。Objective:To study the effect of VECF ASODN and Survivin ASODN co transfection on'Tea8113 tonguecancer cells in nude mice.Methods;25 female mude mice were divided into 5 groups with 5 mice in each group.①In thecombined ASODN group,66μg survivin ASODN and 66μg VECF ASODN were injected into the tumor bodly and periti-moral area of nude mice.The volume of injection was 200μl.It contains lipofectaminetm 2000 and serum-free 1640 solu-tion.②VECF ASODN group:VECF ASODN 66μg was injected in the same way.3 Survivin ASODN group;survivinASODN 66μg was injectedl in the same way.④Liposome group;the same amount of liposome 200μl was injected in thesame way.⑤NS group;the same amount of ns 200μl was injected.Once every 3 days,7 times in total.Two days after theend of the experiment,the mude mice were killed under general anesthesia.The weight of the tumor was measured and the size of the tumor was measured.The tumor was made into paraffin sections.The expression levels of VECF,survivin,PC-NA and CD34 were measured by immunohistochemistry.The microvessel density(MVD)and PCNA label index(PLI)ofthe tumor were calculated.Results:The weight,volume,VECF and survivin protein,PCNA and CD34 expression of xeno-grafts in mude mice were significantly decreased in the combined ASODN group.Conclusion:Co transfection of VECFASODN and Survivin ASODN can significantly inhibit the expression of VECF and Survivin,and inhibit the growth and po-liferation of Tca8113 tongue cancer cells.

关 键 词：血管内皮细胞生长因子 生存素 反义寡核苷酸 裸鼠 舌鳞状细胞癌 

分 类 号：R739.86[医药卫生—肿瘤]