CCL2 Enhances the Viability of Human Chorionic Trophoblast Cell Line HTR-8/SVneo Cells by Inhibiting Interleukin-24 Expression  

作　　者：Jun Shao Yin-Yan He Da-Jin Li Ming-Qing Li 

机构地区：[1]Laboratory for Reproductive Immunology,Hospital and Institute of Obstetrics and Gynecology,Fudan University,Shanghai 200011,China [2]Department of Obstetrics and Gynecology,Shanghai General Hospital,Shanghai Jiao Tong University,Shanghai 200080,China [3]Key Laboratory of Reproduction Regulation of NPFPC,Shanghai 200032,China

出　　处：《Reproductive and Developmental Medicine》2017年第4期198-203,共6页生殖与发育医学（英文版）

基　　金：This study was supported by the National Natural Science Foundation of China(NSFC)(81571509,31671200);the Program for Zhuoxue of Fudan University.

摘　　要：Background:To investigate the regulatory effect of interleukin-24(IL-24)on cell viability of human chorionic trophoblast cell line(HTR-8/SVneo cells).Methods:Immunohistochemical staining was used to detect the expression of IL-24 and its receptors IL-20R1,IL-20R2,and IL-22R1 in villus tissue at early normal pregnancy.The effect of thymic stromal lymphopoietin(TSLP)and chemokine CCL2 on the expression of IL-24 in human chorionic trophoblast cell line HTR-8/SVneo cells was analyzed by In-cell Western.In addition,the effect of recombinant human IL-24(rhIL-24)and CCL2 on the viability of HTR-8/SVneo cells was analyzed by MTT assay.Results:IL-24 and its receptors showed a strong positive staining in trophoblasts at early normal pregnancy.Compared with control group,expression of IL-24 in HTR-8/SVneo cells was significantly inhibited after in vitro stimulation of recombinant human CCL2 protein(rhCCL2)(P<0.001).The viability of HTR-8/SVneo cells was significantly decreased after treatment with rhIL-24(P<0.001).In contrast,anti-IL-24 neutralizing antibody significantly enhanced the viability of HTR-8/SVneo cells(P<0.01).In addition,rhCCL2(100μg/L);enhanced the viability of HTR-8/SVneo cells(P<0.01)in vitro,but this effect was inhibited by treatment with rhIL-24.Conclusions:CCL2 enhances the viability of human trophoblast cell line HTR-8/SVneo cells in vitro by inhibiting the secretion of IL-24,which may be beneficial to blastocyst implantation and placental development.

关 键 词：CCL2 Cell Viability Chorionic Trophoblasts HTR-8/SVneo Cells INTERLEUKIN-24 

分 类 号：R73[医药卫生—肿瘤]