基于提高乙醇产率的常压室温等离子体微藻诱变育种  

Mutation breeding of microalgae by atmospheric and room temperature plasma based on improving ethanol yield

作　　者：孙哲 孙昕[1] 李鹏飞 刘明文 李盟 张清宇 SUN Zhe;SUN Xin;LI Peng-fei;LIU Ming-wen;LI Meng;ZHANG Qing-yu(School of Environment and Municipal Engineering,Xi'an University of Architecture and Technology,Xi'an 710055,China)

机构地区：[1]西安建筑科技大学环境与市政工程学院,陕西西安710055

出　　处：《中国环境科学》2021年第8期3733-3739,共7页China Environmental Science

基　　金：国家“十三五”国家重点研发计划(2016YFC0701001-02);陕西省科技统筹创新工程计划项目(2015KTCL-03-15)。

摘　　要：以普通小球藻FACHB-25为研究对象,利用常压室温等离子体在不同功率条件下对其诱变处理,在功率100W、120W条件下筛选出3株优势藻株,依次编号为S100-7、S120-4、S120-9.其中S120-9藻株培养末期生物量增加明显且多糖产量是原始藻株产量的1.34倍,达到237.98mg/L;S120-4碳水化合物含量占比为37.55%,较原始藻株提高了43.48%.对比了各藻株在光合性能、细胞组分以及细胞形态等方面差异.通过同步糖化发酵(SSF),碳水化合物含量最高的S120-4藻株乙醇产量达到1.58g/10g藻,但其生物量积累受到限制.考虑生物量积累情况,推算出S120-9藻株单位体积藻液乙醇产量最高达到0.1033g/L.Chlorella vulgaris FACHB-25 was mutated by atmospheric and room temperature plasma under different power conditions.Three dominant strains were screened out under the power of 100 W and 120 W,and they were numbered S100-7,S120-4 and S120-9 in turn.Among them,the biomass of the S120-9 algae strain increased significantly at the end of the period,and the polysaccharide production was 1.34 times that of the original algae strain,reaching 237.98 mg/L;the carbohydrate content of S120-4 accounted for 37.55%,which was 43.48%higher than the original algae strain.The differences of photosynthetic performance,cell composition and cell morphology among different strains were compared.Through simultaneous saccharification and fermentation(SSF),the ethanol yield of S120-4 strain with the highest carbohydrate content reached 1.58 g/10 g,but its biomass accumulation was limited.Considering the biomass accumulation,the highest ethanol yield per unit volume of S120-9 was 0.1033 g/L.

关 键 词：常压室温等离子体 小球藻 碳水化合物 生物乙醇 

分 类 号：X172[环境科学与工程—环境科学]