应用16SrRNA基因测序技术研究慢性鼻窦炎微生物学的进展  

Advances in the study of chronic sinusitis microbiology using 16SrRNA gene sequencing technology

作　　者：刘肖 刘红兵[1] 李春花 LIU Xiao;LIU Hongbing;LI Chunhua

机构地区：[1]南昌大学第二附属医院耳鼻咽喉头颈外科,南昌330006

出　　处：《临床耳鼻咽喉头颈外科杂志》2021年第7期658-661,共4页Journal of Clinical Otorhinolaryngology Head And Neck Surgery

摘　　要：慢性鼻窦炎(CRS)定义为一种鼻窦黏膜慢性炎症性疾病,其病程超过12周,是耳鼻咽喉头颈外科的常见病,中国人群中CRS总体患病率约为8%[1]。CRS会引起鼻塞、流涕、面部胀痛、嗅觉下降或丧失等明显身体不适,这些症状可持续存在,严重影响患者生活、工作,给社会经济造成极大的负担[2]。尽管进行了大量研究,但CRS的病因仍不完全清楚,其涉及环境、宿主、微生物等多种因素[3]。Traditional conventional bacterial culture techniques can cultivate fewer types of bacteria, aiming to isolate and identify specific pathogens, guide antibiotic treatment and eradicate pathogens.Macrogenomics techniques can simultaneously identify both cultured and uncultured bacteria in the flora, providing the possibility for the study of symbiotic flora. With the vigorous development of SrRNA and application of 16 gene sequencing technology, the microbial ecology research of chronic sinusitis(chronic rhinosinusitis, CRS) has once again become a hot topic.And the CRS view of microbial community composition and microbial diversity were presented. This review describes the use of 16 SrRNA gene sequencing in recent years advances in technical studies CRS microbial ecology.

关 键 词：鼻窦炎 微生物学 16SRRNA基因 高通量测序 

分 类 号：R765.4[医药卫生—耳鼻咽喉科]