Transgenic overexpression of steroid sulfatase alleviates cholestasis  

作　　者：Mengxi Jiang Meishu Xu Songrong Ren Kyle W.Selcer Wen Xie 

机构地区：[1]Center for Pharmacogenetics,Department of Pharmaceutical Sciences,University of Pittsburgh,Pittsburgh,PA,USA [2]Department of Biological Sciences,Duquesne University,Pittsburgh,PA,USA [3]Department of Pharmacology&Chemical Biology,University of Pittsburgh,Pittsburgh,PA,USA

出　　处：《Liver Research》2017年第1期63-69,共7页肝脏研究（英文）

基　　金：This work was supported in part by the National Institutes of Health grants DK083952 and HD073070(to W.X.).

摘　　要：Background and Aim:Sulfotransferase(SULT)-mediated sulfation and steroid sulfatase(STS)-mediated desulfation represent two critical mechanisms that regulate the chemical and functional homeostasis of endogenous and exogenous molecules.STS catalyzes the hydrolysis of steroid sulfates to form hydroxysteroids.Oxygenated cholesterol derivative oxysterols are known to be endogenous ligands of the liver X receptor(LXR),a nuclear receptor with anti-cholestasis activity,whereas the sulfated oxysterols antagonize LXR signaling.The conversion of sulfated oxysterols to their non-sulfated counterparts is catalyzed by STS.The aim of this study is to determine whether STS can alleviate cholestasis by increasing the activity of LXR.Methods:Liver-specific STS transgenic mice were created and subject to the lithocholic acid(LCA)-induced model of cholestasis.Results:Transgenic overexpression of STS in the liver promoted bile acid elimination and alleviated LCAinduced cholestasis.The protective effect of the STS transgene was associated with the activation of LXR and induction of LXR target genes,likely because of the increased conversion of the antagonistic oxysterol sulfates to the agonistic oxysterols.Conclusions:STS has a novel function in controlling the homeostasis of bile acids by regulating endogenous LXR ligands.

关 键 词：Steroid sulfatase Bile acid TOXICITY CHOLESTASIS Liver X receptor 

分 类 号：O62[理学—有机化学]