鸡胸软骨糖/肽水解物的结构表征及其抗炎活性  

Structural Characterization and Anti-inflammatory Activity of Chicken Breast Cartilage Polysaccharide/Peptide Hydrolysate

作　　者：王瑞琦 郭玉杰 沈青山 刘云鹤 刘济千 张志强 张春晖[2] WANG Rui-qi;GUO Yu-jie;SHEN Qing-shan;LIU Yun-he;LIU Ji-qian;ZHANG Zhi-qiang;ZHANG Chun-hui(College of Food and Wine,Ningxia University,Yinchuan750021,China;Comprehensive Key Laboratory of Agro-Products Processing,Ministry of Agriculture,Institute of Food Science and Technology,Chinese Academy of Agricultural Sciences,Beijing 100193,China;Xinjiang Taikun Group Co.Ltd.,Changji 831100,China;Shandong Haiyu Biotechnology Co.Ltd.,Jining 272000,China)

机构地区：[1]宁夏大学食品与葡萄酒学院,宁夏银川750021 [2]中国农业科学院农产品加工研究所,农业部农产品加工综合性重点实验室,北京100193 [3]新疆泰昆集团股份有限公司,新疆昌吉831100 [4]山东海钰生物技术股份有限公司,山东济宁272000

出　　处：《现代食品科技》2021年第8期44-52,207,共10页Modern Food Science and Technology

基　　金：国家农业科技创新工程项目(CAAS-ASTIP)。

摘　　要：本研究从鸡胸软骨水解物中分离得到胶原蛋白肽和硫酸软骨素并对其进行了结构表征和抗炎活性研究。胶原蛋白肽和硫酸软骨素得率分别为68.32%和18.82%(以干重计)。氨基酸分析表明胶原蛋白肽中甘氨酸含量最高,达14.05 mg/g,其次为谷氨酸(9.09 mg/g)和脯氨酸(7.44 mg/g);紫外光谱分析与圆二色性结果显示所提取样品符合胶原蛋白肽的典型特征;通过高效液相色谱分析发现92.38%的胶原蛋白肽分子量小于1000 u。傅里叶红外光谱、琼脂糖凝胶电泳、二糖组成分析结果表明提取的硫酸软骨素主要由硫酸软骨素A组成,相对纯度为91.87%。通过建立关节炎大鼠模型并检测糖/肽水解物对炎症因子TNF-α、IL-1β、PGE-2水平影响,与模型组相比(TNF-α39.90 pg/mL、IL-1β22.38 pg/mL、PGE-289.41 pg/mL),灌胃软骨水解物、胶原蛋白肽和硫酸软骨素能显著降低关节炎大鼠TNF-α、IL-1β、PGE-2水平(p<0.05)。本实验结果表明,使用软骨水解物、胶原蛋白肽和硫酸软骨素能够抑制炎症因子TNF-α、IL-1β、PGE-2水平,减轻关节炎大鼠炎症反应。The collagen peptides and chondroitin sulfate were isolated from chicken breast cartilage hydrolysate,and their structures and anti-inflammatory activities were investigated in this study.The yields of collagen peptide and chondroitin sulfate were 68.32%and 18.82%(dry weight basis).The amino acid analysis showed that the content of glycine in the collagen peptide was the highest(14.05 mg/g),followed by glutamate(9.09 mg/g)and proline(7.44 mg/g);The results of UV spectroscopy and circular dichroism analyses showed that the extracted samples were conformed to the typical characteristics of collagen peptide;Analysis by high performance liquid chromatography revealed that 92.38%of collagen peptides had a molecular weight smaller than 1000 u.Fourier infrared spectroscopy,agarose gel electrophoresis and disaccharide composition analysis showed that the extracted chondroitin sulfate was mainly composed of chondroitin sulfate A,with a relative purity of 91.87%.By establishing a rat model of arthritis,the effects of polysaccharide/peptide hydrolysate on the levels of inflammatory factors,TNF-α,IL-1β,PGE-2 in rats with arthritis were examined,compared with the model group(TNF-α39.90 pg/mL,IL-1β22.38 pg/mL,PGE-289.41 pg/mL),intragastric cartilage hydrolysate,collagen peptide and chondroitin sulfate could significantly reduce the levels of TNF-α,IL-1βand PGE-2 in arthritic rats(p<0.05).The results of this experiment showed that cartilage hydrolysate,collagen peptides and chondroitin sulfate can be used to inhibit the levels of inflammatory factors,TNF-α,IL-1βand PGE-2,and reduce the inflammatory response in arthritic rats.

关 键 词：软骨水解物 胶原蛋白肽 硫酸软骨素 抗炎活性 

分 类 号：TS251.94[轻工技术与工程—农产品加工及贮藏工程]