Middle ear microbiome differences in indigenous Filipinos with chronic otitis media due to a duplication in the A2ML1 gene  

作　　者：Regie Lyn P.Santos-Cortez Diane S.Hutchinson Nadim J.Ajami Ma.Rina T.Reyes-Quintos Ma.Leah C.Tantoco Patrick John Labra Sheryl Mae Lagrana Melquiadesa Pedro Erasmo Gonzalo d.V.Llanes Teresa Luisa Gloria-Cruz Abner L.Chan Eva Maria Cutiongco-de la Paz John W.Belmont Tasnee Chonmaitree Generoso T.Abes Joseph F.Petrosino Suzanne M.Leal Charlotte M.Chiong 

机构地区：[1]Department of Molecular and Human Genetics,Center for Statistical Genetics,Baylor College of Medicine,Houston,TX 77030,USA [2]Department of Molecular Virology and Microbiology,Alkek Center for Metagenomics and Microbiome Research(CMMR),Baylor College of Medicine,Houston,TX 77030,USA [3]Philippine National Ear Institute,University of the Philippines Manila–National Institutes of Health(UPM-NIH),Manila 1000,Philippines [4]Department of Otorhinolaryngology,University of the Philippines College of Medicine–Philippine General Hospital,Manila 1000,Philippines [5]Institute of Human Genetics,UPM-NIH,Manila 1000,Philippines [6]Philippine Genome Center,University of the Philippines,Diliman,Quezon City 1101,Philippines [7]Departments of Molecular and Human Genetics and Pediatrics,Baylor College of Medicine,Houston,TX 77030,USA [8]Division of Pediatric Infectious Disease and Immunology,Department of Pediatrics,University of Texas Medical Branch,Galveston,TX 77555,USA [9]Current affiliation:Department of Otolaryngology,University of Colorado School of Medicine,Aurora,CO 80045,USA [10]Current address:Illumina,Inc,San Diego,CA 92122,USA

出　　处：《Infectious Diseases of Poverty》2016年第1期889-897,共9页贫困所致传染病（英文）

基　　金：supported by:the National Organization for Hearing Research Foundation,the Hearing Health Foundation,and Action On Hearing Loss(to R.L.P.S.C.);the University of the Philippines Manila–National Institutes of Health(to G.T.A.);the Albert and Margaret Alkek Foundation(to J.F.P.);the United States National Institutes of Health-National Institute on Deafness and Other Communication Disorders grants K18 DC013564(to T.C.),R01 DC011651 and R01 DC003594(to S.M.L.)and R01 DC015004(to R.L.P.S.C.).

摘　　要：Background:Previously rare A2ML1 variants were identified to confer otitis media susceptibility in an indigenous Filipino community and in otitis-prone US children.The goal of this study is to describe differences in the middle ear microbiome between carriers and non-carriers of an A2ML1 duplication variant that increases risk for chronic otitis media among indigenous Filipinos with poor health care access.Methods:Ear swabs were obtained from 16 indigenous Filipino individuals with chronic otitis media,of whom 11 carry the A2ML1 duplication variant.Ear swabs were submitted for 16S rRNA gene sequencing.Results:Genotype-based differences in microbial richness,structure,and composition were identified,but were not statistically significant.Taxonomic analysis revealed that the relative abundance of the phyla Fusobacteria and Bacteroidetes,and genus Fusobacterium were nominally increased in carriers compared to non-carriers,but were non-significant after correction for multiple testing.We also detected rare bacteria including Oligella that was reported only once in the middle ear.Conclusions:These findings suggest that A2ML1-related otitis media susceptibility may be mediated by changes in the middle ear microbiome.Knowledge of middle ear microbial profiles according to genetic background can be potentially useful for therapeutic and prophylactic interventions for otitis media and can guide public health interventions towards decreasing otitis media prevalence within the indigenous Filipino community.

关 键 词：A2ML1 Indigenous population MICROBIOME Middle ear Oligella Otitis media Philippines 

分 类 号：R73[医药卫生—肿瘤]