铁皮石斛花多酚氧化酶提取工艺的优化  

Optimization of Extraction Process of Polyphenol Oxidase from Dendrobium Officinale

作　　者：张珍林[1,2,3] 耿曾奇 关利 曾谦 夏瑜 陈双 ZHANG Zhen-lin;GENG Zeng-qi;GUAN Li;ZENG Qian;XIA Yu;CHEN Shuang(College of Biotechnology and Pharmaceutical Engineering,West Anhui University,liu'an 237012,China;West Anhui Biotechnology Research Center of Natural Medicine and Traditional Chinese Medicine,West Anhui University,liu'an 237012,China;Anhui Engineering Laboratory for Conservation and Sustainable Utilization of Traditional Chinese Medicine Resources,liu'an 237012,China)

机构地区：[1]皖西学院生物与制药工程学院,安徽六安237012 [2]植物细胞工程安徽省工程技术研究中心,安徽六安237012 [3]安徽省中药资源保护与持续利用工程实验室,安徽六安237012

出　　处：《枣庄学院学报》2021年第5期87-92,共6页Journal of Zaozhuang University

基　　金：皖西学院国家级大学生创新训练计划项目(项目编号:202010376061).

摘　　要：用铁皮石斛干花作为提取多酚氧化酶(polyphenoloxidase,PPO)的原料,邻苯二酚作为底物,在460nm下使用分光光度法探究缓冲液pH值、底物的浓度、反应的温度对该酶活性的影响,采用正交分析试验改良提取铁皮石斛干花多酚氧化酶的条件.实验表明:PPO的最优提取工艺条件是,缓冲液的pH是7.0,底物的浓度是0.03mol/L,温度是35℃.The catalytic activity of polyphenol oxidase(PPO)from flowers of Dendrobiumofficinaleowards the substrate catechol was spectrometrically investigated at460nm under the influences of pH,substrateconcentration,temperature.orthogonal experiment was used to optimize the extraction conditions of polyphenol oxidase(PPO)in flowers of DendrobiumOfficinale,The experimental results showed that the optimal extraction conditions of PPO were as follows:the buffer pH value 7.0,the catechol concentration assubstrate0.03mol/L,and the extraction temperature 35℃.

关 键 词：铁皮石斛花 多酚氧化酶 提取 工艺优化 

分 类 号：TS201.2[轻工技术与工程—食品科学]