栀子苷的提取及酶促水解生产京尼平的研究  

Study on Extraction of Geniposide and Its Enzymatic Hydrolysis to Genipin

作　　者：李曦杰 邓月峰 王举政 阳杰 黄晗 李大力[1] LI Xi-jie;DENG Yue-feng;WANG Ju-zheng;YANG Jie;HUANG Han;LI Da-li(Department of Bioengineering, Nanjing University of Science & Technology, Nanjing Jiangsu 210094, China)

机构地区：[1]南京理工大学生物工程系,江苏南京210094

出　　处：《大连民族大学学报》2021年第3期211-213,共3页Journal of Dalian Minzu University

基　　金：国家级大学生创新创业训练计划资助项目(201910288018Z)。

摘　　要：栀子苷是栀子果实中的主要生物活性物质之一。本研究用乙醇浸提栀子果实获得栀子苷浸取液,使用AB-8大孔吸附树脂对栀子苷进行纯化,栀子苷的收率为1.79%。用固定化β-葡萄糖苷酶对纯化后的栀子苷进行酶促水解,获得富含京尼平的栀子苷酶促水解液,经TLC和HPLC分析结果表明:酶法水解12 h后可得到含大量京尼平的水解液。Geniposide is one of the main bioactive substances in the fruit of Gardenia jasminoides.In this study,geniposide was extracted from the fruits of Gardenia jasminoides with 50%ethanol,and purified by AB-8 macroporous resin.The yield of the geniposide was 1.79%.The geniposide was hydrolyzed by immobilizedβ-glucosidase to genipin.The results of TLC and HPLC showed that the hydrolysate containing a large amount of genipin could be obtained after enzymatic hydrolysis for 12 hours.

关 键 词：栀子苷 京尼平 酶促水解 

分 类 号：Q814[生物学—生物工程]