Engineering Corynebacterium glutamicum for Geraniol Production  

作　　者：Man Li Shuo Xu Wenyu Lu 

机构地区：[1]School of Chemical Engineering and Technology,Tianjin University,Tianjin 300072,China [2]Key Laboratory of System Bioengineering(Tianjin University),Ministry of Education,Tianjin 300072,China [3]SynBio Research Platform,Collaborative Innovation Center of Chemical Science and Engineering(Tianjin),Tianjin 300072,China

出　　处：《Transactions of Tianjin University》2021年第5期377-384,共8页天津大学学报（英文版）

基　　金：supported by the National Natural Science Foundation of China (No. 21878220)。

摘　　要：Geraniol is a monoterpenoid alcohol with various applications in food,cosmetics,and healthcare.Corynebacterium glutamicum is a potential platform for terpenoids production because it harbors the methylerythritol phosphate pathway.To engineer C.glutamicum to produce geraniol,two different truncated geraniol synthases (GESs) were respectively expressed,and strain LX02 expressing the truncated GESs from Valeriana officinalis (t Vo GES) produced 0.3 mg/L of geraniol.Then,three geranyl diphosphate synthases (GPPSs) were combinatorially co-expressed with t Vo GES to improve geraniol production.The amounts of produced geraniol were all higher than that produced by strain LX02.Strain LX03 co-expressing ERG20 F96W–N127W (ERG20 WW) and t Vo GES produced the highest amount,5.4 mg/L.Subsequently,the co-overexpression of1-deoxy-D-xylulose-5-phosphate synthase (dxs) and isopentenyl diphosphate isomerase (idi) further increased the production to 12.2 mg/L in strain LX03.Lastly,the production of geraniol was increased to 15.2 mg/L via fermentation optimization.To our knowledge,this is the first report on the engineering of C.glutamicum to produce geraniol and thus can serve as a reference for other monoterpenoid production studies.

关 键 词：Corynebacterium glutamicum GERANIOL Geraniol synthase OVEREXPRESSION Metabolic engineering 

分 类 号：TQ923[轻工技术与工程—发酵工程]