液液萃取-超高效液相色谱-串联质谱法快速检测原料乳中18种喹诺酮药物残留  

Simultaneous rapid determination of 18 quinolones residues in raw milk by liquid-liquid extraction and ultra performance liquid chromatography tandem mass spectrometry

作　　者：周艳华[1] 李涛 潘小红 向俊 ZHOU Yan-hua;LI Tao;PAN Xiao-hong;XIANG Jun(Changsha Environmental Protection College,Changsha,Hunan 410111,China;Hunan Provincial Food Quality Supervision and Inspection Institute,Hunan Provincial Key Laboratory of Food Safety Monitoring and Early Warning,Changsha,Hunan 410111,China;Hunan Institute for Pharmaceutical Inspection,Changsha,Hunan 410001,China)

机构地区：[1]长沙环境保护职业技术学院,湖南长沙410111 [2]湖南省食品质量监督检验研究院食品安全监测与预警湖南省重点实验室,湖南长沙410111 [3]湖南省药品检验研究院,湖南长沙410001

出　　处：《食品与机械》2021年第8期63-69,76,共8页Food and Machinery

基　　金：湖南创新型省份建设专项(编号:2020SK2128);湖南省科技创新平台与人才计划项目(编号:2019TP1058)。

摘　　要：目的:建立一种同时检测原料乳中18种喹诺酮类药物残留的快速检测方法。方法:采用液液萃取净化法进行前处理,并将该前处理技术与超高效液相色谱串联质谱相结合,分析原料乳中18种喹诺酮类药物的残留量。结果:18种喹诺酮在2.0~100.0 ng/mL内具有良好的线性关系(R^(2)≥0.992),检出限为0.02~0.50μg/kg,定量限为0.06~1.50μg/kg;18种喹诺酮的加标回收率为74.0%~106.5%,相对标准偏差为1.1%~13.0%。结论:该方法简单高效、准确度高,可作为原料乳中喹诺酮类药物残留的定量确证检验方法。Objective:A rapid method for simultaneous determination of 18 quinolones residues in raw milk was established.Methods:The raw milk was pretreated by liquid-liquid extraction,and the residues of 18 quinolones in raw milk were analyzed by ultra-performance liquid chromatography-tandem mass spectrometry.Results:The linearity of all the 18 quinolones from 2.0 to 100.0 ng/mL had correlation coefficients greater than 0.992.The limits of detection(LOD)of this method ranged from 0.02 to 0.50μg/kg,and the limits of quantification(LOQ)were 0.06 to 1.50μg/kg.The average recoveries of the 18 quinolones were 74.0%to 106.5%,with the relative standard deviation(n=6)of 1.1%~13.0%.Conclusion:The method is simple,efficient and accurate,and can be used for the quantitative confirmation of quinolones residues in raw milk.

关 键 词：液液萃取 超高效液相色谱-三重四级杆质谱 喹诺酮类化合物 原料乳 

分 类 号：TS252.7[轻工技术与工程—农产品加工及贮藏工程] O657.63[轻工技术与工程—食品科学与工程]