凝集素亲和法在糖蛋白癌症生物标志物分析和检测中的应用  

Application of Lectin Affinity Methods in Analysis and Detection of Glycoprotein Cancer Biomarkers

作　　者：李萍 许文辉 张大伟[1] 贾琼[1] LI Ping;XU Wen-Hui;ZHANG Da-Wei;JIA Qiong(College of Chemistry,Jilin University,Changchun 130012,China)

机构地区：[1]吉林大学化学学院,长春130012

出　　处：《分析化学》2021年第9期1451-1460,共10页Chinese Journal of Analytical Chemistry

基　　金：吉林省科技厅自然科学基金项目(No.20190201079JC)资助。

摘　　要：糖蛋白在调节生物体内各种生理过程中具有重要作用,异常的蛋白质糖基化通常与癌症等疾病的发生和发展有关,许多糖蛋白可作为癌症早期诊断或预后监测的生物标志物。在进行糖蛋白质谱分析鉴定前,从复杂生物样品中有效分离富集糖蛋白是必要的步骤。凝集素对某些特定的糖蛋白具有选择性,凝集素亲和法被广泛用于糖蛋白的分离富集。本文以4种发病率最高的癌症(乳腺癌、肺癌、结直肠癌、前列腺癌)为例,综述了凝集素亲和法与质谱结合在筛选和分析糖蛋白癌症生物标志物中的应用。Glycoproteins play an important role in regulating various physiological processes of organisms.Abnormal protein glycosylation is usually associated with the formation and evolution of diseases such as cancer.Many specific glycoproteins can be used as the biomarkers for early cancer diagnosis or prognosis monitoring.Before identifying the glycoproteins with mass spectrometry,effective separation and enrichment of glycoproteins from complex biological samples is a necessary step.Lectins are selective for certain specific glycoproteins,and lectin affinity methods have been widely used in the separation and enrichment of glycoprotein.By taking the four kinds of cancers(breast cancer,lung cancer,colorectal cancer and prostate cancer)with the highest incidence as examples,this article reviews the applications of lectin affinity methods integrated with mass spectrometry in screening and analyzing cancer biomarkers.

关 键 词：凝集素 糖蛋白 癌症 生物标志物 评述 

分 类 号：R730.43[医药卫生—肿瘤]