Selection of a DNA aptamer for the development of fluorescent aptasensor for carbaryl detection  

作　　者：Yuan Liu Gaojian Yang Taotao Li Yan Deng Zhu Chen Nongyue He 

机构地区：[1]State Key Laboratory of Bioelectronics,School of Biological Science and Medical Engineering,Southeast University,Nanjing 210096,China [2]Hunan Provincial Key Lab of Dark Tea and Jin-Hua,School of Materials and Chemical Engineering,Hunan City University,Yiyang 413000,China [3]Hunan Key Laboratory of Biomedical Nanomaterials and Devices,Hunan University of Technology,Zhuzhou 412007,China

出　　处：《Chinese Chemical Letters》2021年第6期1957-1962,共6页中国化学快报（英文版）

基　　金：financially supported by the National Key Research and Development Program of China(No.2018YFC1602905);the National Natural Science Foundation of China(Nos.61527806 and 61871180)。

摘　　要：An improved ssDNA library immobilized systematic evolution of ligands by enrichment(SELEX)was applied to select aptamers against carbaryl.After nine selection rounds,a highly enriched ssDNA pool was obtained.The Apta3 was demonstrated as the optimal aptame r.In order to facilitate the modification of aptamer,the Apta3 was further truncated with the dissociation constant(K_(d))of 0.364±0.055μmol/L and a fluorescent aptasensor was developed.The linear range for carbaryl was from 100 nmol/L to1500 nmol/L,with the limit of detection was as low as 15.23 nmol/L.Besides,the biosensor was validated for the carbaryl spiked real samples,and the recoveries were between 97.7%and 107.3%.

关 键 词：APTAMER Fluorescence aptasensor SELEX CARBARYL DETECTION 

分 类 号：TS207.53[轻工技术与工程—食品科学] TP212[轻工技术与工程—食品科学与工程]