凌霄花多酚微波提取工艺优化及其抗氧化研究  

Study on Microwave Extraction Process of Polyphenols from Campsis Radicans(L.)and Its Antioxidant Activity

作　　者：郑茜 杨俊 张富贵 郝新才 赵永恒[1,2,3] ZHENG Xi;YANG Jun;ZHANG Fu-gui;HAO Xin-cai;ZHAO Yong-heng(School of Pharmaceutical Sciences,Hubei University of Medicine,Shiyan 442000,China;Hubei Key Laboratory of Wudang Local Chinese Medicine Research,Hubei University of Medicine,Shiyan 442000,China;Hubei Provincial Technology and Research Center for Comprehensive Development of Medicinal Herbs,Hubei University of Medicine,Shiyan 442000,China)

机构地区：[1]湖北医药学院药学院,湖北十堰442000 [2]湖北医药学院武当特色中药研究湖北省重点实验室,湖北十堰442000 [3]湖北医药学院湖北省药用植物综合利用工程技术研究中心,湖北十堰442000

出　　处：《化学试剂》2021年第9期1275-1280,共6页Chemical Reagents

基　　金：湖北省教育厅科学技术研究项目(B2019105、B2020104);十堰市科技局科研项目(19Y21);湖北省科学技术厅重点研发项目(2020BCB040);2018年湖北省高校优秀中青年科技创新团队项目(T201813);湖北医药学院学生科研基金资助项目(2019103)。

摘　　要：以凌霄花为原料,在单因素考察的基础上,考察乙醇浓度、微波功率、提取时间和液料比等因素,通过响应面法优化微波辅助提取凌霄花多酚的工艺,并研究其抗氧化活性。结果表明,微波提取凌霄花多酚的最优工艺为:乙醇浓度40%、微波功率281 W、提取时间37 s、液料比32∶1(mL/g),在此条件下凌霄花多酚的平均提取率为8.07%,与预测值相对标准偏差(2.58%)较为接近。优化的提取工艺科学可行,重现性良好,适用于凌霄花多酚的提取,可为凌霄花的开发利用提供参考。抗氧化研究表明,凌霄花具有较强的抗氧化作用,为凌霄花多酚天然抗氧剂的研发提供了基础。Using Campsis radicans(L.)as raw material,the influence of ethanol concentration,microwave power,extraction time and liquid to solid ratio on the yield of polyphenols in Campsis radicans(L.),are evaluated by single factor test and Box-Behnken response surface method.And antioxidant activity of polyphenols from Campsis radicans(L.)was studied.The results showed that the optimal process was as below:ethanol content 40%,microwave power 281 W,extraction time 37 s,liquid-solid ratio 32∶1(mL/g).Under these extraction process parameters,the average extraction yield of polyphenols was 8.07%,and the relative standard deviation between the predicted values was 2.58%,which was close to the predicted value.The optimized extraction technique is stable and feasible,which can provide a reference for the development and utilization of Campsis radicans(L.).Antioxidant studies have shown that Campsis radicans(L.)has a good antioxidant effect,which provides a basis for the research and development of natural antioxidants of Campsis radicans(L.)polyphenol.

关 键 词：凌霄花 多酚 微波提取 Box-Behnken响应面法 抗氧化活性 

分 类 号：R284.2[医药卫生—中药学]