南京市肠道感染性腹泻样本中食源性致病菌分离及耐药监测分析  

Isolation and drug resistance analysis of food-borne pathogens from intestinal samples of infectious diarrhea in Nanjing

作　　者：金萍[1] 江晓[1] 叶艳华[1] 刘品 王燕[1] 吴正超 JIN Ping;JIANG Xiao;YE Yan-hua;LIU Pin;WANG Yan;WU Zheng-chao(Nanjing Center for Disease Control and Prevention,Nanjing,Jiangsu 210003,China;不详)

机构地区：[1]南京市疾病预防控制中心,江苏南京210003 [2]南京大学医学院附属鼓楼医院,江苏南京210008

出　　处：《中国临床研究》2021年第8期1077-1080,共4页Chinese Journal of Clinical Research

基　　金：江苏省卫生厅预防医学基金科研课题(Y2018003);南京市卫生局“十二五”重大专项(ZDX12006)。

摘　　要：目的分析南京市食源性致病菌对常用抗生素的耐药情况,对食源性疾病的预防及临床合理用药的指导提供依据。方法采用微量肉汤稀释法,对于2019年1月至12月南京市食源性疾病患者腹泻便标本中分离的食源性致病菌(沙门菌、副溶血性弧菌、5种致泻大肠埃希菌共计85株)进行药敏鉴定。结果 85株菌株在药敏实验前,进行生化鉴定菌株复核均阳性,35株沙门菌、36株副溶血性弧菌、14株致泻大肠埃希菌多重耐药率分别为65.7%、0、78.6%;沙门菌中发生耐受的抗生素主要有红霉素(100.0%)、氨苄西林/舒巴坦(65.7%)、头孢唑啉(65.7%)、萘啶酸(65.7%)、氨苄西林(62.9%);副溶血性弧菌中发生耐受的抗生素主要有头孢唑啉(100.0%)、氨苄西林(11.1%)、庆大霉素(2.8%);致泻性大肠埃希菌发生耐受的抗生素主要有红霉素(100.0%)、萘啶酸(71.4%)、氨苄西林(64.3%)、氨苄西林/舒巴坦(57.1%)、阿奇霉素(57.1%)。结论南京市食源性疾病患者粪便标本中分离到的食源性致病菌中致泻大肠埃希菌及沙门菌存在多种抗生素耐药,应进一步加强监测。及时了解南京市食源性致病菌分离及耐药情况,以指导本地区合理使用抗菌药。Objective To analyze the antibiotic resistance of food-borne pathogens in Nanjing and provide the basis for the prevention of food-borne diseases and the guidance of clinical rational drug use.Methods The broth microdilution method was used to identify the drug sensitivity of 85 strains of Salmonella,Vibrio parahaemolyticus and diarrheogenic Escherichia coli isolated from diarrhea samples of food-borne diseases patients treated in Nanjing from January 2019 to December 2019.Results Before drug sensitivity test, 85 strains were confirmed to be positive by biochemical identification, and the multiple drug resistance rates of Salmonella(35 strains),Vibrio parahaemolyticus(36 strains) and diarrheogenic Escherichia coli(14 strains) were 65.7%,0% and 78.6% respectively.The main antibiotics in drug-resistant strain of salmonella were erythromycin(100.0%),ampicillin/sulbactam(65.7%),cefazolin(65.7%) and nalidixic acid(65.7%),ampicillin(62.9%).Cefazolin(100.0%),ampicillin(11.1%) and gentamicin(2.8%) were the main antibiotics in drug-resistant strain of Vibrio parahaemolyticus.The main antibiotics in drug-resistant strain of diarrheogenic Escherichia coli were erythromycin(100.0%),nalidixic acid(71.4%),ampicillin(64.3%),ampicillin/sulbactam(57.1%),azithromycin(57.1%).Conclusions Multiple antibiotic resistance in diarrheogenic Escherichia coli and Salmonella isolated from fecal samples of food-borne diseases patients should be continuously monitored to understand the isolation and drug resistance of food-borne pathogens in Nanjing and to guide the rational use of antibiotics in this area.

关 键 词：食源性致病菌 肉汤稀释法 沙门菌 副溶血性弧菌 致泻大肠埃希菌 耐药性 

分 类 号：R446.5[医药卫生—诊断学]