Antipsychotics preserve telomere length in peripheral blood mononuclear cells after acute oxidative stress injury  

作　　者：Gabriel B.Polho Giancarlo M.Cardillo Daniel S.Kerr Thais Chile Wagner F.Gattaz Orestes V.Forlenza Helena P.Brentani Vanessa J.De-Paula 

机构地区：[1]Laboratório de Neurociências(LIM-27),Departamento e Instituto de Psiquiatria,Hospital das Clinicas HCFMUSP,Faculdade de Medicina,Universidade de São Paulo,SP,Brasil [2]Laboratório de Psicobiologia(LIM-23),Departamento e Instituto de Psiquiatria,Hospital das Clinicas HCFMUSP,Faculdade de Medicina,Universidade de São Paulo,SP,Brasil

出　　处：《Neural Regeneration Research》2022年第5期1156-1160,共5页中国神经再生研究（英文版）

基　　金：supported by grants from FAPESP(Fundacao de AmparoàPesquisa de Sao Paulo,Grant no 2016/01302-9 and 2014/27129-6);CAPES(Coordenacao de Aperfeicoamento de Pessoal de Nível Superior)88887.463672/2019-00。

摘　　要：Antipsychotics may prolong or retain telomere length,affect mitochondrial function,and then affect the metabolism of nerve cells.To validate the hypothesis that antipsychotics can prolong telomere length after oxidative stress injury,leukocytes from healthy volunteers were extracted using Ficoll-Histopaque density gradient.The mononuclear cells layer was resuspended in cell culture medium.Oxidative stress was induced with hydrogen peroxide in cultured leukocytes.Four days later,leukocytes were treated with aripiprazole,haloperidol or clozapine for 7 days.Real-time PCR revealed that treatments with aripiprazole and haloperidol increased the telomere length by 23%and 20%in peripheral blood mononuclear cells after acute oxidative stress injury.These results suggest that haloperidol and aripiprazole can reduce the damage to telomeres induced by oxidative stress.The experiment procedure was approved by the Ethics Committee of Faculty of Medicine of the University of Sao Paulo(FMUSP/CAAE approval No.52622616.8.0000.0065).

关 键 词：ANTIPSYCHOTIC ARIPIPRAZOLE cell culture CLOZAPINE HALOPERIDOL oxidative stress peripheral blood mononuclear cell telomere length 

分 类 号：R96[医药卫生—药理学]