“困难序列”多肽的合成方法  

Synthesis methods of peptides containing"difficult sequences"

作　　者：许小玲 张荣江 谢欣 易小庆 陈伟铭 张剑[2,3] 江峰 XU Xiao-ling;ZHANG Rong-jiang;XIE Xin;YI Xiao-qing;CHEN Wei-ming;ZHANG Jian;JIANG Feng(Gannan Medical University;School of Pharmacy,Gannan Medical University;Key Laboratory of Ministry of Education for Cardiovascular and Cerebrovascular Disease Prevention and Control,Gannan Medical University,Ganzhou,Jiangxi 341000)

机构地区：[1]赣南医学院 [2]赣南医学院药学院 [3]赣南医学院心脑血管疾病防治教育部重点实验室,江西赣州341000

出　　处：《赣南医学院学报》2021年第8期858-864,共7页JOURNAL OF GANNAN MEDICAL UNIVERSITY

基　　金：国家自然科学基金项目(21961003);江西省自然科学基金项目(20192BAB2015114);江西省“双千计划”人才项目(jxsq2018106059);赣南医学院高层次人才启动基金项目。

摘　　要：固相合成是多肽合成中的一种重要方法。固相多肽合成(Solid Phase Peptide Synthesis,SPPS)因避免了液相合成中冗长的重结晶或分离步骤,减少了合成生产过程中的损失,而得到广泛的应用。然而,一些包含较多疏水性氨基酸且容易出现β-折叠等二级结构的“困难序列”多肽仍然难以顺利合成。本文对这些“困难序列”形成的原因、解决方法以及一些经典的“困难序列”多肽的合成策略进行了综述。Solid phase synthesis is an important method for peptide synthesis.Solid phase peptide synthesis(SPPS)has been widely used because it avoids the cumbersome recrystallization or separation steps in liquid phase synthesis and reduces the loss in the synthesis process.However,the"difficult sequences"peptide containing more hydrophobic amino acids and prone to secondary structures,such as β-folding,are still difficult to synthesize.Herein,the forming reasons,solutions for the"difficult sequences"peptides and synthetic strategies of some classical"difficult sequences"peptides are reviewed.

关 键 词：固相多肽合成 Β-折叠 困难序列 

分 类 号：R914.5[医药卫生—药物化学]