探讨IL-18BP对卵巢癌的保护作用及可能被调控的机制  

The protective effect of IL-18BP on ovarian cancer and the possible regulation mechanism

作　　者：秦爱玲 梁丹[1] Qin Ailing;Liang Dan(The First Affiliated Hospital of Guangxi University of Traditional Chinese Medicine,Nanning 530023,Guangxi,China)

机构地区：[1]广西中医药大学第一附属医院,广西南宁530023

出　　处：《右江民族医学院学报》2021年第4期476-480,共5页Journal of Youjiang Medical University for Nationalities

摘　　要：目的探讨IL-18BP对卵巢癌的保护作用及可能被调控的机制。方法利用TCGA和GTEx数据库,分析卵巢癌中IL-18BP表达水平与临床指标信息之间的相关性及对预后的影响;Cox比例风险回归模型分析影响患者预后的因素;GSEA基因集富集分析IL-18BP对卵巢癌细胞功能的影响;Pearson分析与PD-L1表达的相关性。结果与正常卵巢组织相比,肿瘤组织中IL-18BP呈低表达趋势(P<0.05);Kaplan-Meier生存分析结果显示IL-18BP与患者生存率呈有关(P<0.05);多因素Cox分析结果显示IL-18BP为独立保护性预后因素(HR=0.75,P<0.05),年龄为独立危险性预后因素(HR=1.02,P<0.05);χ^(2)检验结果分析显示:IL-18BP的表达和肿瘤分期有关(P<0.05);GSEA富集分析结果显示IL-18BP基因高表达的样本在细胞凋亡以及细胞因子受体相互作用等基因集中显著富集(P<0.05,FDR<0.05);Pearson分析结果显示在卵巢癌中PD-L1与IL-18BP的基因表达水平具有显著的正相关性(R=0.58,P<0.05)。结论IL-18BP对卵巢癌具有保护作用,这种保护作用可能与PD-L1调控IL-18BP的表达有关。Objective To investigate the protective effect of IL-18BP on ovarian cancer and the possible mechanism being regulated.Methods TCGA and GTEx databases were adopted to analyze the correlation between IL-18BP expression in ovarian cancer and the clinical indicators as well as its effect on prognosis.Cox proportional hazards regression model was used to analyze the factors affecting the prognosis of patients.GSEA was adopted to analyze the effect of IL-18BP on the function of ovarian cancer cells.Pearson analysis was conducted to reveal the correlation between IL-18BP and PD-L1 expressions.Results Compared with that of the normal ovarian tissue,the expression of IL-18BP in tumor tissue was lower(P<0.05).Kaplan-Meier survival analysis showed that IL-18BP was related with the survival rate of patients(P<0.05).Multivariate Cox analysis showed that IL-18BP was an independent protective factor for prognosis(HR=0.75,P<0.05),while age was an independent risk prognostic factor(HR=1.02,P<0.05).The results ofχ^(2) test showed that the expression of IL-18BP was related with tumor stages(P<0.05).The expression of IL-18BP was negatively correlated with tumor stages(P<0.05).GSEA analysis showed that the samples with high expression of IL-18BP gene were significantly enriched in the gene sets with apoptosis as well as the gene sets with interacted cytokine receptors(P<0.05,FDR<0.05).Pearson analysis showed that there was a significant positive correlation between PD-L1 and IL-18BP expression in ovarian cancer(R=0.58,P<0.05).Conclusion IL-18BP has protective effect on ovarian cancer,which may be related to the regulation of IL-18BP expression by PD-L1.

关 键 词：卵巢肿瘤 IL-18BP PD-L1 

分 类 号：R737.31[医药卫生—肿瘤]