Synergistic curative effect of chicory extract and cisplatin against thioacetamide-induced hepatocellular carcinoma  

作　　者：Hanaa A.Hassan Hanaa M.Serag Nabil M.Abdel-Hamid Mahmoud M.Amr 

机构地区：[1]Department of Zoology,Division of Physiology,Faculty of Science,Mansoura University,Mansoura 35516,Egypt [2]Biochemistry Department,Pharmacy College,Kafr El-Sheikh University,Kafr El-Sheikh 33516,Egypt

出　　处：《Hepatoma Research》2015年第1期147-154,共8页肝癌研究（英文版）

摘　　要：Aim:Hepatocellular carcinoma(HCC)is the dominant form of primary liver cancer and is histologically and etiologically distinct from other forms of primary liver cancer.The objective of this study was to elucidate the synergistic effect and the role of chicory extract[inulin(IN)]as a chemo-sensitizer for cisplatin(CIS)treatment of HCC.Methods:Five groups of rats were treated for 4 months.These groups consisted of the control group,a group receiving thioacetamide(TAA)(200 mg/kg b.w)in drinking water,a group injected intraperitoneally with a single dose of CIS(7.5 mg/kg b.w)in addition to TAA for 4 months,a group receiving oral doses of IN(10 mg/kg b.w)in addition to TAA for 4 months,and a group injected intraperitoneally with a single dose of CIS(7.5 mg/kg b.w)and IN(10 mg/kg b.w)plus TAA for 4 months.Results:The current data exhibited increment of serum and liver enzyme(alanine aminotransferase,aspartate aminotransferase,alkaline phosphatase,creatine kinase,and lactate dehydrogenase)activity,serum lipid profi le levels(total lipids,total cholesterol,triglycerides,low-density lipoprotein,and very low-density lipoprotein),and a signifi cant increase inα-fetoprotein and bilirubin,accompanied with reduced total serum protein and albumin levels in a HCC rat model.Histopathologically,numerous alterations were detected in hepatic tissues of HCC rats,such as lymphocytic cell infi ltration,damage of hepatocytes,dilated congested central vein with degenerated endothelial cells,and congested blood sinusoids in addition to Masson’s trichrome staining blue collagen fi bers in hepatocytes and central vein indicating hepatic fi brosis.Treatment of HCC rats with CIS or IN improved such deleterious effects,where IN is more effective than CIS,and the best effect can be observed in rats that received both CIS and IN.Conclusion:It could be concluded that IN in chicory extract acts as a chemo-sensitizer to CIS for treatment in an HCC rat model.

关 键 词：CHICORY CISPLATIN hepatocellular carcinoma INULIN 

分 类 号：R73[医药卫生—肿瘤]