NAF与EGCG联合应用对变异链球菌的作用研究  

Effects of combined application of NAF and EGCG on Streptococcus mutans

作　　者：刘晓璇 方圆 王瑞[1,2] LIU Xiaoxuan;FANG Yuan;WANG Rui(Department of Preventive Dentistry,Hospital of Stomatology,Jilin University,Changchun 13000,China)

机构地区：[1]吉林大学口腔医院口腔预防科,吉林长春13000 [2]吉林省牙发育及颌骨重塑与再生重点实验室,吉林长春13000

出　　处：《口腔医学》2021年第8期673-677,共5页Stomatology

基　　金：吉林省卫生计生科技项目(2017W001);吉林省卫生健康研究项目(2020JK001);吉林省卫生适宜技术的应用与推广(2017FP024)。

摘　　要：目的研究氟化钠(sodium fluoride,NAF)与表没食子儿茶素没食子酸酯(Epigallocatechingallate,EGCG)联合应用对变异链球菌的影响。方法采用微量稀释法分别测量NAF与EGCG对变异链球菌的最小细菌抑制浓度MIC值,采用棋盘微量稀释法测量NAF与EGCG联合作用于变异链球菌的MIC值,并计算FIC指数。结果NAF单独作用于变异链球菌MIC值为100.000 mg/L,EGCG单独作用于变异链球菌的MIC值为500.00 mg/L,NAF与EGCG联合应用时MIC值分别为25.000 mg/L和62.50 mg/L,FIC指数为0.375,小于0.500。结论NAF与EGCG联合应用具有协同抑制变异链球菌的作用。Objective To study the effects of the combined application of sodium fluoride(NAF)and Epigallocatechin gallate(EGCG)on Streptococcus mutans.Methods The minimum inhibitory concentration(MIC)of EGCG and NAF against Streptococcus mutans was determined respectively by microdilution method.The potential synergistic/antagonistic effects of the NAF/EGCG combination on the growth of Streptococcus mutans was determined by checkerboard microdilution assays to obtain the FIC index.Results EGCG inhibited growth of Streptococcus mutans planktonic cells was at an MIC of 100.000 mg/L and NAF inhibited growth of Streptococcus mutans planktonic cells was at an MIC of 500.00 mg/L.When NAF and EGCG were used in combination,the MIC values were 25.000 mg/L and 62.50 mg/L respectively.The fraction of inhibitory concentration(FIC)of the NAF/EGCG combination against Streptococcus mutans was 0.375,less than 0.500.Conclusion It is shown that the combined effect of NAF and EGCG has a synergistic inhibitory effect on Streptococcus mutans planktonic cells.

关 键 词：氟化钠 表没食子儿茶素没食子酸酯 变异链球菌 联合药敏实验 

分 类 号：R780.2[医药卫生—口腔医学]