Identification of a novel hydrolase encoded by hy-1 from Bacillus amyloliquefaciens for bioremediation of carbendazim contaminated soil and food  

作　　者：Ying Li Miaomiao Chi Xizhen Ge 

机构地区：[1]Beijing Key Laboratory of Biomass Waste Resource Utilization,Biochemical Engineering College,Beijing Union University,Beijing 100023,China

出　　处：《International Journal of Agricultural and Biological Engineering》2019年第2期218-224,共7页国际农业与生物工程学报（英文）

基　　金：This work was financially supported by Joint Funding of Beijing Municipal Natural Science Foundation-Beijing Municipal Education Commission(KZ201911417049);National Key R&D Program of China(2017YFD0201105);Premium Funding Project for Academic Human Resources Development in Beijing Union University(BPHR2017DZ07).

摘　　要：Carbendazim(MBC)is an effective antifungal and antibacterial pesticide in agricultural applications.However,the MBC-contaminated soil and food are difficult to be restored.In this work,a novel MBC-hydrolase HY-1 encoded by gene hy-1 from an isolated MBC-degrading bacteria Bacillus amyloliquefaciens has been screened and identified.The 858 bp hydrolase gene was expressed in E.coli BL21 and the 32 kDa hydrolase HY-1 was purified.The purified HY-1 was able to catalyze MBC into 2-aminobenzimidazole(2-AB)without the need for any cofactors.Then bioremediation experiment was conducted and both the strain Car4 and cell crude extract of E.coli(pET-hy1)accelerated MBC degradation in soil.Moreover,purified HY-1 was available in removing MBC residue on the surface of cucumber.This work explored the possibility of microbial and enzymatic bioremediation on MBC-contaminated soil and food,provide a new way for bioremediation of pesticide contaminations.

关 键 词：CARBENDAZIM BIOREMEDIATION antifungal and antibacterial pesticide HYDROLASE soil FOOD pesticide contamination 

分 类 号：S48[农业科学—农药学]