酶法辅助聚乙二醇-200提取野菊花中绿原酸的工艺及数学模型分析  

Optimization and Mathematical Model Analysis on Extraction of Chlorogenic Acid from Chrysanthemum indicum by Enzyme-assisted Polyethylene Glycol-200

作　　者：刘霞[1] 方小军 彭敏娟 LIU Xia;FANG Xiao-jun;PENG Min-juan(College of Pharmacy,Gannan Medical University,Ganzhou,Jiangxi 341000,China)

机构地区：[1]赣南医学院药学院,江西赣州341000

出　　处：《粮油食品科技》2021年第5期131-137,共7页Science and Technology of Cereals,Oils and Foods

基　　金：江西省教育厅科技项目(GJJ190801)。

摘　　要：研究了酶法辅助聚乙二醇(PEG)-200提取野菊花中绿原酸的工艺条件。以绿原酸提取率为考察指标,纤维素酶用量、液固比、酶解温度和酶解时间为试验因素,通过单因素试验和响应面法对提取工艺条件进行优化。在此基础上建立了不同液固比下的野菊花中绿原酸提取的4种数学模型。结果表明:最佳工艺为纤维素酶用量0.8%,液固比20∶1(mL∶g),酶解温度55℃,酶解时间2.5 h,在此条件下野菊花绿原酸提取率可达3.92%;Slogistic1模型可以更好地拟合数学模型过程,是描述野菊花中绿原酸提取过程的最佳数学模型。This study analyzes the extraction conditions of enzyme-assisted polyethylene glycol(PEG)-200 extraction of chlorogenic acid from Chrysanthemum indicum.Single factor test and response surface method are used to optimize the extraction process by employing chlorogenic acid extraction rate as inspection index,with cellulase dosage,liquid-solid ratio,enzymolysis temperature and enzymolysis time as test factors.Under the optimized extraction condition,four mathematical models of chlorogenic acid extraction from Chrysanthemum indicum at different liquid-solid ratios are established.The results show that the optimal extraction condition are as follows:0.8% cellulase,with a liquid-solid ratio of 20∶1(mL∶g),at 55℃enzymolysis temperature and of 2.5 hours enzymolysis time.This optimal condition brings up the extraction rate of chlorogenic acid from Chrysanthemum indicum to 3.92%.Slogistic1 model can simulate the mathematical model process and best describe the extraction process of chlorogenic acid from Chrysanthemum indicum.

关 键 词：野菊花 绿原酸 聚乙二醇 酶法 提取 数学模型 

分 类 号：TS201.1[轻工技术与工程—食品科学]