Mucin 1 and interleukin-11 protein expression and inflammatory reactions in the intestinal mucosa of necrotizing enterocolitis children after surgery  

作　　者：Hong-Xia Pan Chang-Song Zhang Chia-Hui Lin Min-Min Chen Xiao-Zhong Zhang Nong Yu 

机构地区：[1]Department of Clinical Laboratory Medicine,Suzhou BenQ Medical Center,Affiliated BenQ Hospital of Nanjing Medical University,Suzhou 215011,Jiangsu Province,China [2]Department of Clinical Laboratory Medicine,The Affiliated Suzhou Science and Technology Town Hospital,Nanjing Medical University,Suzhou 215153,Jiangsu Province,China [3]General Manager's Office,Suzhou Gallant Biotech Biotechnology Co.Ltd,Suzhou 215163,Jiangsu Province,China [4]Department of Cardiology,National High Tech Development Zone Hospital,Suzhou 215129,Jiangsu Province,China [5]Department of Laboratory Medicine,National High Tech Development Zone Hospital,Suzhou 215129,Jiangsu Province,China

出　　处：《World Journal of Clinical Cases》2021年第25期7372-7380,共9页世界临床病例杂志

基　　金：Suzhou Science and Technology Program,No.SLT202005;Suzhou Municipal Commission of Health and Family Planning,No.LCZX202031;Suzhou New District Science and Technology Plan,No.2019Z009;Independent Innovation Project of National High Tech Development Zone Hospital,No.SGY2018C03.

摘　　要：BACKGROUND Necrotizing enterocolitis(NEC)of the newborn is a frequently occurring clinical disease in infants.The mortality rate of NEC in premature infants is as high as 50%,and the morbidity rate is on the rise.NEC has already caused serious impacts on newborn survival and poses serious threats to both children and families.AIM To investigate the expression and significance of mucin 1(MUC1)and interleukin-11(IL-11)in the intestinal mucosa of infants with neonatal NEC after surgery.METHODS Forty-eight postoperative intestinal mucosal specimens from children with NEC(NEC group)and twenty-two intestinal mucosal specimens from children with congenital intestinal atresia(control group)were collected in our hospital.Immunohistochemical staining and Western blot analysis were used to examine the protein expression of MUC-1 and IL-11 in the two groups.The serum levels of tumor necrosis factor-α(TNF-α)and IL-1βin the two groups were measured by enzyme-linked immunosorbent assay,and the relationship between MUC-1 and IL-11 protein expression and serum TNF-αand IL-1βlevels was analyzed by the linear correlation method.RESULTS The protein expression of MUC-1 and IL-11 in the NEC group was significantly lower than that in the control group,and the difference was statistically significant(P<0.05).The levels of serum TNF-αand IL-1βin the NEC group were significantly higher than those in the control group(P<0.05).The protein expression of MUC-1 and IL-11 in the NEC group negatively correlated with serum TNF-αand IL-1βlevels(P<0.05).There was a significant negative correlation between the protein expression of MUC-1 and IL-11 and the levels of serum TNF-αand IL-1βin the NEC group.CONCLUSION The protein expression of MUC1 and IL-11 in the intestinal mucosa of children with NEC is significantly downregulated after surgery.This downregulation may be involved in the pathogenesis of this disease and has a certain correlation with inflammatory response factors in children with NEC.

关 键 词：Neonatal necrotizing enterocolitis Mucin 1 INTERLEUKIN-11 INFLAMMATION Intestinal mucosa Expression 

分 类 号：R726.5[医药卫生—儿科]