MS1 is essential for male fertility by regulating the microsporocyte cell plate expansion in soybean  

作　　者：Xiaolong Fang Xiaoyuan Sun Xiangdong Yang Qing Li Chunjing Lin Jie Xu Wenjun Gong Yifan Wang Lu Liu Limei Zhao Baohui Liu Jun Qin Mengchen Zhang Chunbao Zhang Fanjiang Kong Meina Li 

机构地区：[1]Innovative Center of Molecular Genetics and Evolution,School of Life Sciences,Guangzhou University,Guangzhou 510006,China [2]Soybean Research Institute,National Engineering Research Center for Soybean,Jilin Academy of Agricultural Sciences,Changchun 130033,China [3]State Key Laboratory of Rice Biology,China National Rice Research Institute,Chinese Academy of Agricultural Sciences,Hangzhou 311401,China [4]Core Facility and Technical Service Center for SLSB,School of Life Sciences and Biotechnology,Shanghai Jiao Tong University,Shanghai 200240,China [5]Cereal&Oil Crop Institute,Hebei Academy of Agricultural and Forestry Sciences,Shijiazhuang 050031,China

出　　处：《Science China(Life Sciences)》2021年第9期1533-1545,共13页中国科学（生命科学英文版）

基　　金：supported by the National Natural Science Foundation of China(32072084,31871648,and 31971969)。

摘　　要：Male sterility is an essential trait in hybrid seed production,especially for monoclinous and autogamous food crops.Soybean male-sterile ms1 mutant has been known for more than 50 years and could be instrumental in making hybrid seeds.However,the gene responsible for the male-sterile phenotype has remained unknown.Here,we report the map-based cloning and characterization of the MS1 gene in soybean.MS1 encodes a kinesin protein and localizes to the nucleus,where it is required for the male meiotic cytokinesis after telophase Ⅱ.We further substantiated that MS1 colocalizes with microtubules and is essential for cell plate formation in soybean male gametogenesis through immunostaining.Both ms1 and CRISPR/Cas9 knockout mutants show complete male sterility but are otherwise phenotypically normal,making them perfect tools for producing hybrid seeds.The identification of MS1 has the practical potential for assembling the sterility system and speeding up hybrid soybean breeding.

关 键 词：SOYBEAN male sterility MS1 KINESIN CYTOKINESIS hybrid breeding 

分 类 号：S565.1[农业科学—作物学]