红豆树叶总黄酮提取工艺优化及抗氧化性研究  

Optimization of Extracting Process of Total Flavonoids from the Leaves of Ormosia hosiei and the Antioxidant Activity

作　　者：全颖萱 黄冰冰 贝佳炎 郑威 李林海 倪林[1,2] 徐会有 Quan Yingxuan;Huang Bingbing;Bei Jiayan;Zheng Wei;Li Linhai;Ni Lin;Xu Huiyou(College of Plant Protection,Fujian Agriculture and Forestry University,Fuzhou 350002,China;Conservation and Utilization of Natural Biological Resources Fujian University Engineering Research Center,Fuzhou 350002,China.)

机构地区：[1]福建农林大学植物保护学院,福建福州350002 [2]自然生物资源保育利用福建省高校工程研究中心,福建福州350002

出　　处：《中国野生植物资源》2021年第8期20-26,共7页Chinese Wild Plant Resources

基　　金：国家级大学生创新创业训练计划项目(202010389024);国家级大学生创新创业训练计划项目(202110389002);国家级大学生创新创业训练计划项目(202110389039X);福建省本科教育教学改革项目(FBJG20180211);制药工程学科竞赛指导团队(111416040)。

摘　　要：目的:优化红豆树叶总黄酮提取工艺并研究其抗氧化性。方法:采用超声辅助法分别考察液料比、乙醇浓度和超声时间对红豆树叶总黄酮提取率的影响。以单因素试验为依据,进行响应面设计试验及数据分析,进而对其工艺进行优化设计,同时进行总黄酮的抗氧化性试验。结果:各因素对提取率的影响由大到小顺序为乙醇浓度、液料比和超声时间;且最佳提取工艺为乙醇浓度69.45%,液料比20.15∶1(mL/g),超声时间90.17 min。在此工艺下,总黄酮提取率为23.85%,通过验证试验发现,试验提取率为23.87%,与预测值相对标准偏差为0.06%;同时红豆树叶总黄酮对DPPH自由基和ABTS自由基表现出较好的清除效果。结论:优化后的红豆树叶总黄酮提取工艺稳定可行,并且其具有较强的抗氧化能力。Objective:Optimization of the extraction technology of total flavonoids from leaves of Ormosia hosiei and study on its antioxidant activity.Methods:The effects of liquid to solid ratio,ethanol concen⁃tration and ultrasonic time on the extraction rates of total flavonoids from leaves of O.hosiei were investi⁃gated by ultrasonic-assisted extraction.Based on the results of single-factor test,response surface design and data analysis were carried out to further optimize the ultrasonic-assisted extraction.At the same time,the antioxidant activity of total flavonoids was tested.Results:The influence of factors on flavo⁃noids extraction rate was in turn ethanol concentration,liquid to solid ratio,the ultrasonic time.The opti⁃mal extraction process conditions were liquid to solid ratio20.15∶1(mL/g),ethanol concentration 69.45%and ultrasonic time 90.17 min.Under the above conditions,the extraction of total flavonoids was 23.85%.The experimental results showed that the extraction of total flavonoids was 23.87%,which was close to the predicted value and the relative standard deviation between the two was 0.06%.Meanwhile,finding the total flavonoids from leaves of O.hosiei had good scavenging effects on DPPH free radicals and ABTS free radicals.Conclusion:The optimized extraction process of total flavonoids from the leaves of O.hosiei was stable and workable,and it has a strong antioxidant ability.

关 键 词：红豆树叶 总黄酮 超声辅助提取 响应面法 抗氧化性 

分 类 号：TQ35[化学工程]