非小细胞肺癌MET表达及基因改变与PD-L1表达及免疫微环境的关系  

The relationship of MET expression and gene changes with PD-L1expression and immune microenvironment in non-small cell lung cancer

作　　者：李超[1] 何淼[1] 杨桄权 黄虹超 陈兆红[1] LI Chao;HE Miao;YANG Guangquan;HUANG Hongchao;CHEN Zhaohong(Department of Oncology,People’s Hospital of Deyang City,618000 Deyang,China)

机构地区：[1]德阳市人民医院肿瘤科,618000

出　　处：《免疫学杂志》2021年第9期803-808,共6页Immunological Journal

摘　　要：目的探讨非小细胞肺癌(non-small cell lung cancer,NSCLC)MET表达及基因改变与程序性死亡分子配体1(PD-L1)表达及免疫微环境的关系。方法回顾性分析2019年1月—2020年12月医院收治的112例NSCLC患者,收集病理活检或手术切除组织标本。采用免疫组织化学染色法检测MET、PD-L1表达及CD8^(+)T细胞浸润水平;采用荧光原位杂交法检测MET基因状态;并分析MET表达及基因状态与临床病理特征、PD-L1表达及CD8^(+)T细胞浸润水平关系。结果NSCLC组织MET、PD-L1阳性率显著高于癌旁组织;<65岁、腺癌、临床分期Ⅲ~Ⅳ期、EGFR突变型、淋巴转移者MET阳性率显著升高;NSCLC组织MET基因扩增率为11.61%;腺癌、淋巴转移者MET基因扩增率显著升高;MET表达及MET基因扩增与PD-L1阳性表达有关;校正影响MET表达及基因状态因素后,MET表达及MET基因扩增为影响PD-L1表达的预测因素;NSCLC原发和转移病灶CD8^(+)T细胞浸润率分别为64.29%、39.29%;MET基因扩增与CD8^(+)T细胞浸润水平有关;校正影响MET表达及基因状态因素后,MET基因扩增与CD8^(+)T细胞浸润水平独立相关。结论MET表达尤其是MET基因改变可影响NSCLC中PD-L1表达和免疫微环境,其中MET基因扩增与CD8^(+)T细胞浸润水平独立相关。To investigates the relationship of MET expression and gene changes with programmed deathligand 1(PD-L1)expression and immune microenvironment in non-small cell lung cancer(NSCLC),total of 112 NSCLC patients admitted to our hospital from January 2019 to December 2020 were retrospectively analyzed.All resected pathological or surgical specimens of these patients were collected.Immunohistochemical staining was used to detect the expression of MET,PD-L1 and the level of CD8^(+)T cell infiltration;Fluorescence in situ hybridization was used to detect MET gene status.The relationship of MET expression and gene status with the clinicopathological characteristics,PD-L1 expression and CD8^(+)T cell infiltration level were analyzed.Data showed that the positive rate of MET and PD-L1 in NSCLC tissues were significantly higher than those of adjacent normal tissues(P<0.05).Positive rate of MET was increased significantly in patients of age over 65-year-old,adenocarcinoma,clinical stageⅢtoⅣ,EGFR mutation or lymphatic metastasis(P<0.05).The amplification rate of MET gene was 11.61%in NSCLC tissues,while the amplification rate was significantly higher in patients with adenocarcinoma or lymphatic metastasis(P<0.05).MET expression and MET gene amplification were related to the positive expression of PD-L1(P<0.05).After adjusting the factors affecting MET expression and gene status,we found that MET expression and MET gene amplification were predictive factors of PD-L1 expression(P<0.05);the CD8^(+)T cell infiltration rates of primary and metastatic NSCLC lesions were 64.29%and 39.29%,respectively;MET gene amplification wasrelated to the level of CD8^(+)T cell infiltration(P<0.05);After adjusting the factors affecting MET expression and genestatus,the results showed that MET gene amplification was independently related to the level of CD8^(+)T cellinfiltration(P<0.05).Taken together,the expression of MET,especially the change of met gene,can affect theexpression of PD-L1 and immune microenvironment in NSCLC.And the amplificati

关 键 词：非小细胞肺癌 MET 基因扩增 PD-L1 免疫微环境 

分 类 号：R734.2[医药卫生—肿瘤]