大麻素受体在子宫腺肌病在位内膜中的表达  

Expression of Cannabinoid Receptors in the Eutopic Endometrium in Patients With Adenomyosis

作　　者：汪沙[1] 段华[1] 沈雪 Wang Sha;Duan Hua;Shen Xue(Department of Minimally Invasive Gynecology, Beijing Obstetrics and Gynecology Hospital, Capital Medical University, Beijing 100006, China)

机构地区：[1]首都医科大学附属北京妇产医院妇科微创中心,北京100006

出　　处：《中国微创外科杂志》2021年第9期817-821,共5页Chinese Journal of Minimally Invasive Surgery

基　　金：国家自然科学基金(81571412);首都医科大学附属北京妇产医院中青年学科骨干培养专项(FCYY201920);首都医科大学临床医学高精尖学科建设项目(1192070309)。

摘　　要：目的研究大麻素受体CB1和CB2在子宫腺肌病患者在位内膜及非腺肌病患者子宫内膜组织中表达的差异。方法选择2017年7月~2018年12月因弥漫型子宫腺肌病行全子宫切除术的45例为腺肌病组(增殖期23例,分泌期22例),同期因宫颈疾病切除子宫的34例非子宫腺肌病患者(宫颈上皮内瘤变Ⅲ级16例,宫颈癌ⅠA期18例)作为对照组(增殖期22例,分泌期12例),通过免疫组化二步法和实时荧光定量PCR技术检测2组子宫内膜CB1、CB2蛋白及mRNA的表达水平。结果①CB1蛋白在腺肌病组增殖期、分泌期在位内膜中表达量均低于对照组(均P=0.000)。腺肌病组CB1蛋白在增殖期与分泌期的表达差异无统计学意义(P=0.101),而对照组增殖期低于分泌期(P=0.009)。②CB2蛋白在腺肌病组增殖期、分泌期在位内膜中表达量均低于对照组(P=0.011、0.000)。2组CB2蛋白的表达均在增殖期低于分泌期(均P=0.000)。③CB1 mRNA在腺肌病组增殖期、分泌期在位内膜中的表达量均低于对照组(均P=0.000)。腺肌病组CB1 mRNA在增殖期与分泌期的表达差异无统计学意义(P=0.238),而对照组增殖期低于分泌期(P=0.000)。④CB2 mRNA在腺肌病组增殖期、分泌期在位内膜中的表达量均低于对照组(均P=0.000)。2组CB2 mRNA的表达均在增殖期低于分泌期(均P=0.000)。结论腺肌病子宫在位内膜中大麻素受体CB1和CB2的表达均下调且CB1的表达失去周期性变化,提示大麻素受体可能参与腺肌病的发生发展。Objective To observe the differences of expression of cannabinoid receptors CB1 and CB2 in endometrial samples derived from adenomyosis patients and non-adenomyosis patients.Methods From July 2017 to December 2018,45 cases of diffuse adenomyosis undergoing hysterectomy were enrolled as the adenomyosis group(23 cases in the proliferative phase and 22 cases in the secretory phase).Another 34 cases of non-adenomyosis(16 cases of cervical intraepithelial neoplasia gradeⅢand 18 cases of cervical carcinoma stageⅠA)in the same period were enrolled as the control group(22 cases in the proliferative phase and 12 cases in the secretory phase).The expression levels of protein and mRNA of CB1 and CB2 in the endometrium of the two groups were detected by immunohistochemistry and real-time PCR.Results①The expression levels of CB1 protein in the eutopic endometrium during the proliferation and secretory phases of the adenomyosis group were lower than those of the control group(both P=0.000).The expression of CB1 protein in the endometrium of the adenomyosis group was not significantly different between proliferation and secretion phase(P=0.101).In the control group,the expression of CB1 protein in the endometrium during the proliferation phase was lower than during the secretion phase(P=0.009).②The expression levels of CB2 protein in the eutopic endometrium during the proliferation and secretory phases of the adenomyosis group were lower than those of the control group(P=0.011 and 0.000).The expression levels of CB2 protein in both groups were significantly lower in the proliferation phase of endomtrium than in the secretory phase(both P=0.000).③The expression levels of CB1 mRNA in the eutopic endometrium during the proliferation and secretory phases of the adenomyosis group were lower than those of the control group(both P=0.000).The expression of CB1 mRNA in the adenomyosis group was not significantly different between the proliferation and secretory phases(P=0.238),while in the control group,that during the prolife

关 键 词：子宫腺肌病 大麻素受体 CB1 CB2 子宫内膜 

分 类 号：R73[医药卫生—肿瘤]