The putative elongator complex protein Elp3 is involved in asexual development and pathogenicity by regulating autophagy in the rice blast fungus  

作　　者：ZHANG Li-mei CHEN Shu-ting QI Min CAO Xue-qi LIANG Nan LI Qian TANG Wei LU Guo-dong ZHOU Jie YU Wen-ying WANG Zong-hua ZHENG Hua-kun 

机构地区：[1]State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops,College of Life Sciences,Fujian Agriculture and Forestry University,Fuzhou 350002,P.R.China [2]Fujian Universities Key Laboratory for Plant–Microbe Interaction,College of Life Sciences,Fujian Agriculture and Forestry University,Fuzhou 350002,P.R.China [3]Meishan Vocational and Technical College,Meishan 620010,P.R.China [4]Institute of Oceanography,Minjiang University,Fuzhou 350108,P.R.China [5]National Engineering Research Center of JUNCAO Technology,College of Life Sciences,Fujian Agriculture and Forestry University,Fuzhou 350002,P.R.China

出　　处：《Journal of Integrative Agriculture》2021年第11期2944-2956,共13页农业科学学报（英文版）

基　　金：supported by grants from the National Natural Science Foundation of China(31770156 and 2016YFD0300700);the Innovative Research Funding of FAFU,China(CXZX2018051 and CXZX2018052)。

摘　　要：Autophagy is responsible for maintaining fundamental cellular homeostasis and is,therefore,essential for diverse development processes.This study reported that PoElp3,the putative catalytic subunit of Elongator complex,is involved in the maintenance of autophagy homeostasis to facilitate asexual development and pathogenicity in the rice blast fungus Pyricularia oryzae.It was found that the ΔPoelp3 strains were defective in vegetative growth,conidiation,stress response,and pathogenicity.The mutants exhibited hyper-activated autophagy in the vegetative hyphae under both nutrient-rich and nutrient-deficient conditions.The hyper-activation of autophagy possibly suppressed the production of vegetative hyphae in the ΔPoelp3 strains.Moreover,the ΔPoelp3 strains were found to be more sensitive to rapamycin during vegetative-and invasive-hyphal growth but have no effect on Target-of-Rapamycin(TOR)signaling inhibition.Taken together,these results demonstrated that PoElp3 is involved in asexual development and pathogenicity by regulating autophagy in the rice blast fungus.

关 键 词：elongator ELP3 AUTOPHAGY asexual development PATHOGENICITY Pyricularia oryzae 

分 类 号：S435.111.41[农业科学—农业昆虫与害虫防治]