花生主要过敏原Ara h 1线性B细胞表位的预测及鉴定  

Prediction and Identification of Linear B Cell Epitope on the Major Peanut Allergen Ara h 1

作　　者：王俊娟 李欣芮 陈成 孙善峰 刘桂蓉 车会莲 WANG Junjuan;LI Xinrui;CHEN Cheng;SUN Shanfeng;LIU Guirong;CHE Huilian(Beijing Laboratory for Food Quality and Safety,College of Food Science and Nutritional Engineering,China Agricultural University,Beijing 100083,China)

机构地区：[1]中国农业大学食品科学与营养工程学院,食品质量与安全北京实验室,北京100083

出　　处：《食品科学》2021年第17期106-112,共7页Food Science

基　　金：国家自然科学基金面上项目(31972185)。

摘　　要：花生过敏由于其高致敏率和致敏严重性而引起了人们的广泛关注。Ara h 1是花生中的主要过敏原,属于Cupin超家族,通过抗原表位识别结合免疫球蛋白E引发花生过敏。本研究采用生物信息学分析花生主要过敏原Cupin超家族Ara h 1线性B细胞表位氨基酸组成,及其与Ara h 1二级、三级结构之间关系,通过质谱分析Ara h 1氨基酸序列中的抗消化肽段,并分析抗消化肽段与预测线性B细胞表位的关系。结果表明,Ara h 1的线性B细胞表位富含亲水性氨基酸和带电氨基酸;其二级结构没有明显的分布规律,具有一定的回转折叠结构;分析Ara h 1三级结构发现,表位主要位于单体之间的疏水相互作用区域,部分表位埋入三聚体构象内部;Ara h 1抗消化序列与表位之间存在部分重叠。综上,质谱检测体外模拟胃肠道消化肽段并结合表位生物信息学分析可以作为鉴定Cupin超家族线性B细胞表位的新方法。Recently,there has been widespread concern about peanut allergy because of the high sensitization rate to peanut and peanut-induced anaphylaxis.Ara h 1 is a major allergen in peanut that belongs to the Cupin superfamily and can trigger peanut allergies through recognizing and binding to immunoglobulin E(IgE).In this study,bioinformatics was used to analyze the amino acid composition of the linear B cell epitope on peanut allergen Ara h 1 and its correlation with the secondary and tertiary structures of the allergen.The digestion resistant peptides in the Ara h 1 sequence were analyzed by mass spectrometry(MS),and the relationship between the peptides and the predicted linear B cell epitopes was analyzed.The results showed that the linear B cell epitope on Ara h 1 was rich in hydrophilic and charged amino acids.In the secondary structure,the epitope showed no regular distribution pattern but with tuning and folding conformations.In the tertiary structure,it was mainly located in the hydrophobic interaction area between the monomers,partly buried in the trimer conformation.There was a partial overlap between the digestion resistant peptide sequence and the predicted epitope.Detecting peptides from simulated gastrointestinal digestion in vitro by MS combined with bioinformatics analysis can be used as a new method to identify the linear B cell epitope on the Cupin superfamily.

关 键 词：花生过敏 Ara h 1 线性B细胞表位 抗消化肽 生物信息学分析 

分 类 号：TS201.6[轻工技术与工程—食品科学]