不同季节新鲜鸭蛋表面污染细菌的多样性分析  被引量:6

Diversity and Seasonal Variation of Bacteria Contaminating Fresh Duck Egg Surface

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作  者:卢昌丽 熊香元 龚慧可 任佑华 陈力力[1] 刘焱[1] LU Changli;XIONG Xiangyuan;GONG Huike;REN Youhua;CHEN Lili;LIU Yan(Hunan Province Key Laboratory of Food Science and Biotechnology,College of Food Science and Technology,Hunan Agricultural University,Changsha 410128,China)

机构地区:[1]湖南农业大学食品科学技术学院,食品科学与生物技术湖南省重点实验室,湖南长沙410128

出  处:《食品科学》2021年第18期306-313,共8页Food Science

基  金:湖南省教育厅重点科研项目(16A099);公益性行业(农业)科研专项(201303084);湖南省教育厅一般项目(20C0959)。

摘  要:为研究鸭蛋壳表面污染细菌的多样性以及季节性变化,在春、夏、秋和冬4个季节采集新鲜鸭蛋,利用超声波洗涤蛋壳制备细菌样本,通过Illumina MiSeq高通量测序分析样品细菌菌群的多样性。结果表明,共获得697244条优化序列归属于650个操作分类单元(operational taxonomic units,OTU),包括35个门,298个属,447个种。其中相对丰度占比较大的优势菌有:厚壁菌门(Firmicutes)芽孢杆菌属(Bacillus)和肠球菌属(Enterococcus),分别占比17.78%和17.16%;变形菌门(Proteobacteria)肠杆菌科(Enterobacteriaceae)埃希氏菌属-志贺菌属(Escherichia-Shigella)占比17.14%。4个季节样品的优势菌群相同,皆为芽孢杆菌属(Bacillus)、肠球菌属(Enterococcus)和埃希氏菌属-志贺菌属(Escherichia-Shigella),但种群结构和物种丰度各不相同,冬季样本Chao指数和ACE指数最大,平均分别为596.5±22.29和587.5±17.82;Shannon指数最大和Simpson指数最小,平均分别为3.52±1.02和0.1±0.07,种群复杂度表现最为突出;春夏秋冬四季样品共有的OTU为35个,分别占各样品OTU数目的0.06%~70.00%,独有的OTU数目分别为9(春)、0(夏)、17个(秋)和496个(冬),各个季节样品的菌群种类具有较大的差异性;不同季节分别有弧菌(Vibrio)、淤泥假单胞菌(Pseudomonas_caeni)、肠杆菌(Enterobacter)、溶菌杆菌(Lysinibacillus)、梭菌(Clostridium_sensu_stricto_1)等存在,部分致病菌受气温影响明显。该结果为进一步研究鸭蛋主要腐败菌及其控制提供了理论依据。To study the diversity and seasonal variation of bacteria contaminating duck eggshell surfaces,fresh duck eggs collected in different seasons were cleaned by ultrasonic washing to prepare bacterial samples.The diversity of bacterial flora in the samples was analyzed by Illumina MiSeq high-throughput sequencing.The results showed that a total of 697244 optimal sequences were obtained and assigned to 650 operational taxonomic units(OTU),covering 35 phyla,298 genera,and 447 species.For each seasonal samples,the dominant bacteria with a high relative abundance were Bacillus and Enterococcus(17.78%and 17.16%)of the phylum Firmicutes,and Escherichia-Shigella(17.14%)of Enterobacteriaceae in Proteobacteria.However,there was a significant difference in the microbial community structure and species richness among these samples.The maximum Chao and ACE were observed for winter samples(n=4),with averages of 596.5±22.29 and 587.5±17.82,respectively,as well as maximum Shannon’s index and minimum Simpson’s index,3.52±1.02 and 0.1±0.07 on average,respectively,indicating that the species complexity was highest.A total of 35 OTUs were common to the spring,summer,autumn and winter samples,accounting for 0.06%–70.00%of the total number of OTUs in each sample;and 9,0,17 and 496 OTUs were unique to these samples respectively.There were great differences in the composition of the microflora on the surface of eggshells among seasons.Vibrio,Pseudomonas_caeni,Enterobacter,Lysinibacillus,Clostridium_sensu_stricto_1 existed in different seasons.Some pathogenic floras were obviously affected by temperature.These results will provide a theoretical basis for further studies on the major spoilage bacteria of duck eggs and for controlling them.

关 键 词:鸭蛋壳 季节性 高通量测序 细菌多样性 

分 类 号:TS253.1[轻工技术与工程—农产品加工及贮藏工程]

 

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