Combining Metabolic Alkyne Labeling and Click Chemistry for Secretome Analysis of Serum-Containing Conditioned Medium  

作　　者：Jiangnan Zheng Yuan Mao Shun Feng Ruijun Tian 

机构地区：[1]Department of Chemistry,School of Science,Southern University of Science and Technology,Shenzhen,Guangdong,518055 China [2]School of Life Science and Engineering,Southwest Jiaotong University,Chengdu,Sichuan,610031 China [3]Guangdong Provincial Key Laboratory of Cell Microenvironment and Disease Research,Southern University of Science and Technology,1088 Xueyuan Road,Shenzhen,Guangdong,518055 China

出　　处：《Chinese Journal of Chemistry》2021年第7期1843-1848,共6页中国化学（英文版）

基　　金：This work was supported by grants from China State Key Basic Research Program Grants(2016YFA0501403,2016YFA0501404 and 2020YFE0202200);the National Natural Science Foundation of China(31700088);Guangdong Provincial Fund for Distinguished Young Scholars(2019B151502050);Shenzhen Innovation of Science and Technology Commission Grant(JCYJ20170412154126026).

摘　　要：Main observation and conclusion Bioorthogonal click chemistry has emerged as a powerful tool for the specific modification of proteins in complex mixtures.Metabolic labeling of proteins with azide followed by the copper-catalyzed azide−alkyne cycloaddition(CuAAC)with alkyne-based affinity probes/beads is widely applied to study protein turnover and post-translational modifications(PTMs).

关 键 词：Click chemistry Mass spectrometry PROTEOMICS SECRETOME Metabolic labeling 

分 类 号：O62[理学—有机化学]