Mechanistic Study of Oxidoreductase AprQ Involved in Biosynthesis of Aminoglycoside Antibiotic Apramycin  

作　　者：Jinxiu Wang Suze Ma Wei Ding Tuo Chen Qi Zhang 

机构地区：[1]State Key Laboratory of Cryospheric Science,Key Laboratory of Extreme Environmental Microbial Resources and Engineering,Northwest Institute of Eco-Environment and Resources,Chinese Academy of Sciences,Lanzhou,Gansu,730000 China [2]Department of Chemistry,Fudan University,Shanghai,200433 China [3]University of Chinese Academy of Sciences,Beijing,100049 China [4]State Key Laboratory of Microbial Metabolism,School of Life Sciences&Biotechnology,Shanghai Jiao Tong University,Shanghai,200240 China

出　　处：《Chinese Journal of Chemistry》2021年第7期1923-1926,共4页中国化学（英文版）

基　　金：This work is supported in part by grants from the National Key Research and Development Program(Nos.2018Y F A0900402 and 2016Y F A0501302);from the National Natural Science Foundation of China(Nos.21822703 and 21921003);from the MOST Inter-Government International Science and Technology Innovation Cooperation project(No.2019YFE0121100).

摘　　要：Main observation and conclusion The aminoglycoside antibiotic apramycin contains a unique bicyclic octose moiety,and biosynthesis of this moiety involves an oxidoreductase AprQ.Unlike other known“Q”series proteins involved in aminoglycosides biosynthesis,AprQ does not work with an aminotransferase partner,and performs a four-electron oxidation that converts a CH2OH moiety to a carboxylate group.

关 键 词：Biosynthesis FLAVIN FLAVOPROTEIN OXIDASE Enzyme mechanism 

分 类 号：O62[理学—有机化学]