Ni离子螯合亲和层析法分离大叶海藻中的抗氧化多肽  

Separation of Antioxidant Polypeptides from Seaweed by Ni Chelating Affinity Chromatography

作　　者：余科义 徐礼龙 毕静 YU Keyi;XU Lilong;BI Jing(Henan Technical Institute,Zhengzhou 450042;Gushi County Agricultural Product Quality and Safety Testing Center,Xinyang 465200)

机构地区：[1]河南应用技术职业学院,郑州450042 [2]固始县农产品质量安全检测中心,信阳465200

出　　处：《食品工业》2021年第8期122-125,共4页The Food Industry

基　　金：河南省高等学校重点科研项目(21B150008)。

摘　　要：采用Ni2+螯合抗氧化多肽,经亲和层析柱分离纯化的方法提取大叶海藻中的抗氧化多肽。对影响洗脱效果和抗氧化多肽乳化活性的因素进行探讨,并分析抗氧化多肽的洗脱曲线、组成成分和螯合率。对提取的抗氧化多肽的活性在O_(2)-·和·OH清除能力2个方面进行测定。结果表明,Ni离子螯合多肽的亲和层析分离方法有效,可获得较好的多肽纯度和活性。Antioxidant polypeptide was extracted from marine algaeby Ni2+chelated antioxidant polypeptide and separated and purified by affinity chromatography column.The factors influencing elution efficiency and emulsifying activity of antioxidant polypeptide were discussed.Elution curve,composition and chelating rate of antioxidant polypeptide were analyzed.The activity of the antioxidant polypeptide was determined in two aspects:the scavenging ability of O_(2)-·and·OH.The results showed that the affinity chromatography method of Ni2+chelated polypeptide was effective,and it could achieve good purity and activity performance of the polypeptide.

关 键 词：亲和层析 大叶海藻 镍螯合物 抗氧化多肽 

分 类 号：TS201.2[轻工技术与工程—食品科学]