紫外发光二极管对P.deceptionensis CM2杀菌作用及机制  

Effect of Ultraviolet-C Iight-Emitting Diodes on P.deceptionensis CM2:Inactivation Efficiency and Mechanism

作　　者：刘骁 李云菲 王雯雯 相启森 赵卫东 杜桂红 LIU Xiao;LI Yunfei;WANG Wenwen;XIANG Qisen;ZHAO Weidong;DU Guihong(College of Food and Biological Engineering,Zhengzhou University of Light Industry,Zhengzhou 450001;Henan Kaifeng Dayong Industrial Co.,Ltd.,Kaifeng 475200)

机构地区：[1]郑州轻工业大学食品与生物工程学院,郑州450001 [2]河南开封大用实业有限公司,开封475200

出　　处：《食品工业》2021年第8期150-154,共5页The Food Industry

基　　金：“十三五”国家重点研发计划(2018YFD0400603);河南省重点研发与推广专项(192102110106);河南省自然科学基金(202300410497)。

摘　　要：研究紫外发光二极管(UVC-LEDs)处理对假单胞菌CM2(P.deceptionensis CM2)的灭活作用及机理。结果表明,UVC-LEDs处理对P.deceptionensis CM2具有良好灭活效果,且灭活效果随处理剂量升高而增强。处理剂量8 mJ/cm^(2)和4000 mJ/cm^(2)时,纯培养体系和鸡肉表面P.deceptionensis CM2分别降低6.09 log10 CFU/mL和2.45log10 CFU/g。场发射扫描电镜(FE-SEM)结果表明,经UVC-LEDs(4000 mJ/cm^(2))处理后,P.deceptionensis CM2细胞形态发生明显皱缩。随着处理剂量(1000~4000 mJ/cm^(2))的增加,P.deceptionensis CM2细胞中碘化丙啶荧光强度显著增强,胞外核酸和蛋白含量显著增加。以上结果表明,UVC-LEDs杀菌作用可能与细胞结构破坏、细胞膜通透性增加有关。试验结果为UVC-LEDs在食品安全控制和保鲜领域的应用提供参考。This work aimed to investigate the inactivation efficiency and mechanisms of ultraviolet-C light-emitting diodes(UVC-LEDs)against P.diceptionensis CM2.After UVC-LEDs treatment,the populations of P.deceptionensis CM2 significantly decreased with increasing UVC-LEDs dose.At doses of 8 mJ/cm^(2)and 4000 mJ/cm^(2),the numbers of P.deceptionensis CM2 in pure culture system and on chicken breast decreased by 6.09 log10 CFU/mL and 2.45 log10 CFU/g,respectively.As revealed by field emission scanning electron microscopy(FE-SEM),UVC-LEDs treatment at 4000 mJ/cm^(2)resulted in obvious shrink in morphology of P.deceptionensis CM2 cells.With increasing doses of 1000 to 4000 mJ/cm^(2),the propidium iodide fluorescence intensity and the contents of extracellular nucleic acid and protein of P.deceptionensis CM2 significantly increased.In summary,the bactericidal effect of UVC-LEDs may be associated with the changes of cell structure and the membrane permeability.This work provided basis for the application of UVC-LEDs in the food safety control and preservation.

关 键 词：紫外发光二极管 P.deceptionensis CM2 灭活 机理 

分 类 号：TN312.8[电子电信—物理电子学] TS205[轻工技术与工程—食品科学]