LncRNA UCA1通过调节miR-206/PTP1B轴影响宫颈癌细胞增殖迁移及侵袭  

LncRNA UCA1 affects the proliferation,migration and invasion of cervical cancer cells by regulating miR-206/PTP1B axis

作　　者：冯艳[1] 孙延霞 贺晶[1] 李红霞[1] FENG Yan;SUN Yanxia;HE Jing;LI Hongxia(Department of Gynecology,The Affiliated Hospital of Yan'an University,Yan'an 716000,Shannxi,China)

机构地区：[1]延安大学附属医院妇科,陕西延安716000

出　　处：《西部医学》2021年第9期1276-1283,共8页Medical Journal of West China

基　　金：延安市科技惠民计划项目(2017HM-02-02)。

摘　　要：目的探讨LncRNA UCA1通过miR-206/PTP1B轴对宫颈癌细胞增殖、迁移和侵袭的影响。方法将细胞培养后随机分为对照组、si-NC组(转染si-NC)、si-UCA1组(转染si-UCA1)、si-PTP1B组(转染si-PTP1B)、miR-NC组(转染miR-NC)、miR-206组(转染miR-206)、anti-miR-206组(转染anti-miR-206)、miR-NC+si-NC组(转染miR-NC和si-NC)、miR-206+si-NC组(转染miR-206和si-NC)、anti-miR-206+si-NC组(转染anti-miR-206和si-NC)、anti-miR-206+si-UCA1组(转染anti-miR-206和si-UCA1)、miR-NC+si-PTP1B组(转染miR-NC和si-PTP1B)、anti-miR-206+si-PTP1B组(转染anti-miR-206和si-PTP1B)。qRT-PCR检测宫颈癌细胞UCA1和miR-206的表达;Western blot检测宫颈癌细胞PTP1B的表达;荧光素酶报告基因实验验证UCA1与miR-206、miR-206与PTP1B的靶向关系;CCK-8检测宫颈癌细胞活力;流式细胞术检测宫颈癌细胞凋亡;Transwell实验检测宫颈癌细胞迁移和侵袭能力。结果与人正常宫颈上皮细胞相比,宫颈癌细胞中UCA1表达显著升高(P<0.01),miR-206表达显著降低(P<0.05)。与si-NC组相比,si-UCA1组细胞UCA1表达、细胞活力、迁移和侵袭能力均显著降低(P<0.01),细胞凋亡率显著升高(P<0.01)。UCA1和miR-206间存在相互调控作用;与miR-NC+si-NC组相比,miR-206+si-NC组细胞活力、迁移和侵袭能力显著降低(P<0.05),细胞凋亡率显著升高(P<0.01),anti-miR-206+si-NC组则呈现相反变化;与anti-miR-206+si-NC组相比,anti-miR-206+si-UCA1组细胞活力、迁移和侵袭能力显著降低(P<0.01),细胞凋亡率显著升高(P<0.01)。miR-206靶向调控PTP1B;与miR-NC+si-NC组相比,anti-miR-206+si-NC组细胞活力、迁移和侵袭能力显著升高(P<0.05),细胞凋亡率显著降低(P<0.05),miR-NC+si-PTP1B组则呈现相反变化;与anti-miR-206+si-NC组相比,anti-miR-206+si-PTP1B组细胞活力、迁移和侵袭能力显著降低(P<0.05),细胞凋亡率显著升高(P<0.05)。结论LncRNA UCA1通过调节miR-206/PTP1B轴促进宫颈癌细胞增殖、迁移和侵袭。Objective To investigate the effect of LncRNA UCA1 on the proliferation,migration and invasion of cervical cancer cells via miR-206/PTP1B axis.Methods qRT-PCR was used to detect the expression of UCA1 and miR-206 in cervical cancer cells.Western blot was used to detect the expression of PTP1B in cervical cancer cells.luciferase reporter gene experiment was used to verify the targeting relationship between UCA1 and miR-206,miR-206 and PTP1B.CCK-8 was used to detect cervical cancer cell viability;flow cytometry was used to detect cervical cancer cell apoptosis.Transwell test was used to detect cervical cancer cell migration and invasion ability.Results Compared with human normal cervical epithelial cells,UCA1 expression was significantly increased in cervical cancer cells(P<0.01),and miR-206 expression was significantly decreased(P<0.05).Compared with the si-NC group,the UCA1 expression,cell viability,migration and invasion ability of the si-UCA1 group were significantly reduced(P<0.01),and the apoptosis rate was significantly increased(P<0.01).There is a mutual regulation effect between UCA1 and miR-206;compared with miR-NC+si-NC group,the cell viability,migration and invasion ability of miR-206+si-NC group are significantly reduced(P<0.05),and the rate of apoptosis significantly increased(P<0.01),the anti-miR-206+si-NC group showed opposite changes.Compared with the anti-miR-206+si-NC group,the cell viability,migration and invasion ability of the anti-miR-206+si-UCA group were significantly reduced(P<0.01),and the apoptosis rate was significantly increased(P<0.01).Compared with miR-NC+si-NC group,the cell viability,migration and invasion ability of anti-miR-206+si-NC group were significantly increased(P<0.05),and the rate of apoptosis significantly decreased(P<0.05),miR-NC+si-PTP1B group showed opposite changes.Compared with anti-miR-206+si-NC group,cell viability,migration and invasion ability was significantly reduced(P<0.05),and the apoptosis rate was significantly increased(P<0.05).Conclusion LncRNA UCA1 prom

关 键 词：LncRNA UCA1 宫颈癌 增殖、迁移和侵袭 miR-206 PTP1B 

分 类 号：R737.33[医药卫生—肿瘤]