茶叶水提物对高脂饮食诱导小鼠胰岛素抵抗作用的研究  

Effect of tea extract on insulin resistance induced by high-fat diet in mice based on ELISA

作　　者：李解 翟秀明[1,2] 唐敏 张维[3] 袁林颖 侯渝嘉 LI Jie;ZHAI Xiuming;TANG Min;ZHANG Wei;YUAN Linying;HOU Yujia(Tea Research Institute of Chongqing Acedemy of Agricultural Sciences,Chongqing 402160,China;Chongqing Engineering Technology Research Center of Tea Industry,Chongqing 402160,China;Economic Crop Technology Promotion Station of Yongchuan District,Chongqing 402160,China)

机构地区：[1]重庆市农业科学院茶叶研究所,重庆402160 [2]重庆市茶叶工程技术研究中心,重庆402160 [3]重庆市永川区经济作物技术推广站,重庆402160

出　　处：《食品与发酵工业》2021年第18期135-140,共6页Food and Fermentation Industries

基　　金：重庆市永川区科技局自然科学基金计划项目(Ycstc,2019nb0103);重庆市农业科学院农业发展资金项目(NKY-2021AB004);重庆市农业科学院青年创新团队项目(NKY-2019QC05)。

摘　　要：该文研究了茶叶水提物(tea water extract,TAE)对高脂饮食诱导小鼠胰岛素抵抗的作用。将40只雄性C57BL/6小鼠随机分为4组:正常对照组(normal control,NC)、高脂对照组(high fat diet,HF)、正常补充1%(质量分数,下同)TAE组(NC+1%TAE,NT)、高脂补充1%TAE组(HF+1%TAE,HT),NC、NT组给予标准饲料,HF、HT组给予高脂饲料喂养,NT、HT组分别补充1%TAE,喂养13周,测定小鼠体重、空腹血糖及胰岛素水平,基于酶联免疫方法测定小鼠血清丙氨酸转氨酶(alanine aminotransferase,ALT)、天冬氨酸转氨酶(aspartate aminotransferase,AST)、总胆固醇(total cholesterol,TC)、三酰甘油(triglyceride,TG)、胰岛素、肿瘤坏死因子-α、白细胞介素-2、脂多糖(lipopolysaccharide,LPS)等含量,取小鼠附睾白色脂肪组织(epididymal white adipose tissue,Epi-WAT)和肝脏组织分别用苏木精-伊红和油红O染色。结果显示,TAE可显著降低小鼠体重的增加及空腹血糖、胰岛素水平,降低血清中的TC、TG、ALT、AST水平,减少高脂饮食诱导引起的全身低度炎症,改善肠道菌群微生物菌群及代谢,减少Epi-WAT细胞肥大和肝脏脂肪变性。结果表明,TAE能有效地改善血糖和血脂水平,减少肝脏组织中的脂肪累积,预防肝脏脂肪变性,对饮食所致胰岛素抵抗具有较好的调节作用,减少Ⅱ型糖尿病的发生。The aim of this study was to explore the effect of tea water extract(TAE)on insulin resistance induced by a high-fat diet in mice.Forty male C57BL/6 mice were randomly divided into 4 groups:normal control group(NC),high-fat control group(HF),normally supplemented with 1%TAE group(NT)and high-fat supplemented with 1%TAE group(HT).NC and NT groups were given standard feed,HF and HT groups were given high-fat feed,NT and HT groups were fed with 1%(w/w)TAE respectively for 13 weeks.The body weight,fasting blood glucose and insulin levels of mice were measured.Determination of mouse serum alanine aminotransferase(ALT),aspartate aminotransferase(AST),total cholesterol(TC),triacylglycerol(TG),insulin,and tumor necrosis factor-α(TNF-α),interleukin-2(IL-2),lipopolysaccharide(LPS),etc.Mouse epididymal white adipose tissue(Epi-WAT)and liver tissue were stained with H&E and Oil Red O,respectively.The results showed that TAE could significantly reduce weight gain,fasting blood glucose,insulin levels in mice,reduce serum TC,TG,ALT,and AST levels.Low-grade inflammation of the whole body induced by a high-fat diet was reduced.Intestinal flora microflora and metabolism were improved.And Epi-WAT cell hypertrophy and liver steatosis were reduced.The results showed that TAE could effectively improve blood sugar and blood lipid levels and reduce fat accumulation in liver tissue.Moreover,it could prevent liver steatosis and has a good regulatory effect on insulin resistance and type 2 diabetes caused by diet.

关 键 词：茶叶水提物 高脂饮食 小鼠 胰岛素抵抗 

分 类 号：TS201.4[轻工技术与工程—食品科学]