A potential role of karyopherin a2 in the impaired maturation of dendritic cells observed in glioblastoma patients  

作　　者：Konstantinos Gousias Alexander von Ruecker Gerrit H.Gielen Pitt Niehusmann Andreas Waha Hartmut Vatter Matthias Simon 

机构地区：[1]Department of Neurosurgery,University Hospital of Bonn,Sigmund-Freud-Strasse 25,53105 Bonn,Germany [2]Department of Neurosurgery,University Hospital of Bochum,Bergmannsheil,Bürkle-de-la-Camp-Platz 1,44789 Bochum,Germany [3]Department of Pathology,University Hospital of Bonn,Sigmund-Freud-Strasse 25,53105 Bonn,Germany [4]Department of Neuropathology,University Hospital of Bonn,Sigmund-Freud-Strasse 25,53105 Bonn,Germany

出　　处：《Neuroimmunology and Neuroinflammation》2015年第1期8-14,共7页神经免疫与神经炎症（英文版）

摘　　要：Aim:Patients with glioblastomas demonstrate well‑documented immunological impairments including decreased numbers of mature dendritic cells(DCs).Recent data identified karyopherin a2(KPNA2),a nucleocytoplasmic shuttling receptor,as diagnostic and prognostic biomarker for gliomas.The aim of this ongoing study is to correlate parameters of immunity and nucleocytoplasmic transport in glioblastoma patients.Methods:We preoperatively collected serum from 17 patients with glioblastomas and determined DC subsets(HLA DR+Lin-,CD34-,CD45+,CD123+,CD11+were analyzed)using a 6‑color flow cytometry panel.Expression levels of KPNA2 and nuclear accumulation of p53 were evaluated semi‑quantitatively by immunohistochemistry.O6‑methylguanine DNA methyltransferase(MGMT)and isocitrate dehydrogenase‑1(IDH‑1)status were assessed by pyrosequencing and immunohistochemistry,respectively.Results:Median expression levels for both KPNA2 and p53 were 5-10%.IDH‑1‑R132H mutation and MGMT promoter hypermethylation was detected in 3/16 and 1/9 patients,respectively.Mean counts of total mature DCs,myeloid DCs and plasmacytoid DCs were 9.6,2.1,3.4 cells/μL.A preliminary analysis suggests an association between low KPNA2 nuclear expression and increased numbers of mature DCs.However,this correlation did not reach statistical significance so far(P=0.077).Conclusion:Our preliminary data may indicate a role of KPNA2 in the impaired maturation of DCs observed in glioblastoma patients.

关 键 词：GLIOBLASTOMAS isocitrate dehydrogenase‑1 karyopherin a2 mature dendritic cells O6‑methylguanine DNA methyltransferase p53 

分 类 号：R73[医药卫生—肿瘤]