龙安柚花毒理学安全性评价  

Toxicological safety evaluation on the flower of Citrus grandis var.Longanyou

作　　者：李蕊瑞 刘科亮[3] 吴德举 陈锦瑶[1,2] 张立实 LI Rui-Rui;LIU Ke-Liang;WU De-Ju;CHEN Jin-Yao;ZHANG Li-Shi(West China School of Public Health,Sichuan University,Chengdu 610041,China;Sichuan Key Laboratory of Food Safety Monitoring and Risk Assessment,Chengdu 610041,China;Institute of Toxicology,Sichuan Center for Disease Control and Prevention,Chengdu 610041,China;Sichuan Institute of Food Safety,Chengdu 610041,China)

机构地区：[1]四川大学华西公共卫生学院,成都610041 [2]四川省食品安全监测与风险评估重点实验室,成都610041 [3]四川省疾病预防控制中心,毒理检验所,成都610041 [4]四川省食品安全学会,成都610041

出　　处：《食品安全质量检测学报》2021年第16期6586-6593,共8页Journal of Food Safety and Quality

基　　金：四川省科技厅重点研发项目(2019ZYZF0122)~。

摘　　要：目的评估龙安柚花的食用安全性。方法按现行食品安全国家标准进行急性经口毒性试验、细菌回复突变(Ames)试验、哺乳动物红细胞微核试验、体外哺乳类细胞染色体畸变试验、90 d经口毒性试验和致畸试验,并进行统计分析。结果在本试验条件下,SD大鼠和KM小鼠摄入龙安柚花后均未表现出急性经口毒效应,急性经口半数致死量(lethaldose50,LD50)大于10g/(kg·BW),该花属实际无毒级;龙安柚花未引起TA97a、TA98、TA100、TA102、TA1535菌种发生回复突变;哺乳动物红细胞微核试验与体外哺乳类细胞染色体畸变试验结果均为阴性;90 d经口毒性试验中,与对照组相比,各剂量组各性别动物的体重、摄食量、食物利用率、血液学指标、血生化指标和组织病理学检查均未见特异的毒性改变,龙安柚花的未观察到有害作用剂量(no observed adverse effect level,NOAEL)为8 g/(kg·BW);未观察到致畸作用。结论龙安柚花属实际无毒级,本试验条件下龙安柚花无急性毒性、遗传毒性、致畸性和亚慢性毒性。Objective To evaluate the toxicity on the flower of Citrus grandis var.Longanyou.Methods Acute oral toxicity test,acterial revert mutation(Ames)test,mammalian erythrocyte micronucleus test,chromosome aberration test of mammalian cells in vitro,90 d oral toxicity test and teratogenicity test were conducted according to the current national food safety standards.Statistical analysis was conducted.Results In the acute oral toxicity test,there was no acute oral toxic effect on the flower of Citrus grandis var.Longanyou in SD rats and KM mice,and its acute lethal dose 50(LD50)was greater than 10 g/(kg·BW),thus,the flower was classified as the actual non-toxic level.No reverse-mutation of TA97 a,TA98,TA100,TA102,TA1535 were induced by the flower of Citrus grandis var.Longanyou.The results were negative in mammalian erythrocyte micronucleus test as well as chromosome aberration test of mammalian cells in vitro.In the 90-day oral toxicity test,compared with the control group,the weight,food intake,food utilization rate,hematology,blood biochemistry and histopathology of animals of all sexes in each dose group did not manifest toxic effects.No observed adverse effect level(NOAEL)of the flower was 8 g/(kg·BW),and no teratogenic effect was observed.Conclusion The flower of Citrus grandis var.Longanyou is actually non-toxic.Acute toxicity,genetic toxicity,teratogenicity and sub-chronic toxicity are negative under the conditions of these experiments.

关 键 词：龙安柚 柚花 毒理学试验 安全性评价 

分 类 号：TS202.1[轻工技术与工程—食品科学]