纳米氧化锌/葡萄皮红改性大豆分离蛋白膜的制备与性能研究  

Preparation and characterization of soy protein isolate films modified by ZnO nanoparticles and grape-skin red

作　　者：冉锐敏 王璐瑶 何宾宾 何淑蹇 徐鸥 吴钰楼 余惠容 陈赛艳 冀俊杰[2] Ran Ruimin;Wang Luyao;He Binbin;He Sujian;Xu Ou;Wu Yulou;Yu Huirong;Chen Saiyan;Ji Junjie(College of Food Science,Sichuan Agricultural University,Ya an 625014,China;Shandong Technician Institute,Jinan 250200,China)

机构地区：[1]四川农业大学食品学院,雅安625014 [2]山东技师学院,济南250200

出　　处：《农业工程学报》2021年第14期294-301,共8页Transactions of the Chinese Society of Agricultural Engineering

基　　金：四川省科技创新苗子工程资助项目(2021106);四川省科技厅项目(2020YFN0147)。

摘　　要：为改善大豆分离蛋白膜的性能,将纳米氧化锌(ZnO Nanoparticles,ZnO NPs)和葡萄皮红(Grape-Skin Red,GSR)加入大豆分离蛋白(Soy Protein Isolate,SPI)中制备SPI/ZnO NPs/GSR复合膜,对复合膜的性能进行表征。结果表明当葡萄皮红、ZnO NPs和大豆分离蛋白以1∶2∶25的质量比制备复合膜时,相对于SPI/ZnO NPs膜,葡萄皮红可提高ZnO NPs和大豆分离蛋白的相容性,改善ZnO NPs在SPI膜中的分散性,并与ZnO NPs发挥协同作用提高SPI膜的机械性能、耐水性能和热稳定性(P<0.05)。SPI/ZnO NPs/GSR复合膜相比较于SPI膜,拉伸强度从1.37 MPa升至3.28 MPa,熔点从194℃升至231℃,含水率从34.41%降至25.37%,水蒸气透过系数从5.57×10^(-12)(g·cm)/(cm^(2)·s·Pa)降至4.74×10^(-12)(g·cm)/(cm^(2)·s·Pa)。此外,复合膜对金黄色葡萄球菌和大肠杆菌表现出优异的抗菌性能,抑菌圈直径随着活性成分的添加呈上升趋势(大肠杆菌:SPI膜无,SPI/ZnO NPs膜2.29 cm,SPI/ZnO NPs/GSR膜2.36 cm;金黄色葡萄球菌:SPI膜无,SPI/ZnO NPs膜2.32 cm,SPI/ZnO NPs/GSR膜2.42 cm),在活性包装应用中具有极大潜力。研究结果为大豆分离蛋白基薄膜的生产应用提供参考。Soy Protein Isolate(SPI),a byproduct of the soybean oil industry,can widely be expected to serve as one of the best alternatives to synthetic plastics,due mainly to excellent film-forming properties,including biocompatible,biodegradable,environmentallyfriendly,and cheap to produce.Nevertheless,the SPI normally contains a large number of hydrogen,ionic,and hydrophobic bonds within the molecular structure.The SPI films presented relatively low water resistance,mechanical properties,and thermal stability,due to a large number of hydrophilic bonds in the structures(-OH,-NH2,-COOH,-SH),compared with conventional synthetic plastic films.Therefore,it is urgent to explore protein-based plastic films with improved functional properties.ZnO Nanoparticles(NPs)are highly functional nano-scale products with several attractive properties,such as non-toxic,large specific surface area,strong UV protection,antibacterial properties,and high thermal stability.As such,ZnO NPs can be incorporated into the SPI film matrix,further enhancing the physical and antibacterial properties of films.Furthermore,the presence of N-H groups in the protein chain can interact with ZnO NPs through hydrogen bonds.Auxiliary agents are also needed to integrate the SPI molecules and ZnO NPs,because the high energy,unsaturation,and instability of ZnO NPs can cause the surface roughness of the film.Alternatively,a by-product of grape processing,Grape-Skin Red(GSR)is generally used as a natural anthocyanin pigment.A typical C6-C3-C6 carbon skeleton structure in GSR also contains two benzoyl rings and oxygenated hexameric heterocycles with cations(a typical 2-phenyl-benzopyran cation structure).The structural homogeneity and surface roughness of film can be improved via the cross-linking of highly reactive structures with SPI molecules through hydrogen bonds and electrostatic interactions.These interactions also ensure the uniform mixing of ZnO NPs and SPI.GSR also contains rich and free phenolic hydroxyl structures that can offer the SPI film considerable a

关 键 词：膜 机械性能 抗菌性能 大豆分离蛋白膜 纳米氧化锌 葡萄皮红 

分 类 号：TS206.4[轻工技术与工程—食品科学]