Gracilaria fisheri oligosaccharides ameliorate inflammation and colonic epithelial barrier dysfunction in mice with acetic acid-induced colitis  

作　　者：Brenda Siringoringo Nawiya Huipao Chittipong Tipbunjong Jongdee Nopparat Santad Wichienchot Albert M.Hutapea Pissared Khuituan 

机构地区：[1]Division of Health and Applied Sciences,Faculty of Science,Prince of Songkla University,Songkhla,Thailand [2]Gut Biology and Microbiota Research Unit,Prince of Songkla University,Songkhla,Thailand [3]Trace Analysis and Biosensor Research Center,Prince of Songkla University,Songkhla,Thailand [4]Center of Excellence in Functional Foods and Gastronomy,Faculty of Agro-Industry,Prince of Songkla University,Songkhla,Thailand [5]Department of Pharmacy,Faculty of Science,Universitas Advent Indonesia,Bandung,Indonesia

出　　处：《Asian Pacific Journal of Tropical Biomedicine》2021年第10期440-449,共10页亚太热带生物医学杂志（英文版）

基　　金：supported by Prince of Songkla University(grant number SCI6202058S);Thailand Education Hub-ASEAN Countries(TEH-AC)scholarship(grant number TEH AC 012/2017);Prince of Songkla University,Songkhla,Thailand.

摘　　要：Objective:To investigate the effect of Gracilaria fisheri oligosaccharides(GFO)on inflammation and colonic epithelial barrier dysfunction in colitis mice.Methods:The animals were treated by oral gavage with distilled water,1000 mg/kg inulin,100,500,or 1000 mg/kg GFO for 14 d,or treated with 50 mg/kg mesalamine for 5 d after colitis induction(on day 10).Histopathology,inflammatory cytokines,colonic permeability,and tight junction proteins were investigated by hematoxylin and eosin staining,immunohistochemical staining,Ussing chamber technique,and Western blotting assays,respectively.Results:GFO ameliorated histological damage in colitis mice when compared to untreated colitis mice.Treatments with 100,500,and 1000 mg/kg GFO reduced TNF-αexpression,while IL-1βwas significantly reduced in colitis mice treated with 500 and 1000 mg/kg.Compared to untreated colitis mice,GFO increased transepithelial electrical resistance,reduced fluorescein isothiocyanate-dextran paracellular flux,and modulated tight junction proteins(occludin and claudin 2)in colitis mice.Conclusions:GFO has anti-inflammatory activity and could modulate colonic epithelial barrier dysfunction in acetic acid-induced colitis mice.Furthermore,GFO could modulate the expression of tight junction proteins that play important roles in colonic barrier function.

关 键 词：Ulcerative colitis Cytokines Gut permeability Tight junction Red algae Gracilaria fisheri MICE ANTI-INFLAMMATION Colonic epithelial barrier 

分 类 号：R574.62[医药卫生—消化系统]