乙酰肝素酶通过转录因子Runx2调控多发性骨髓瘤细胞功能  

Heparanase Regulates Multiple Myeloma Cell Function through the Transcription Factor Runx2

作　　者：潘倩影[1] 水炜 牛媛媛[2] 张常然[2] Pan Qianying;Shui Wei;Niu Yuanyuan(Department of Hematology,The First Affiliated Hospital of Sun Yat-sen University,Guangdong 510080,China)

机构地区：[1]中山大学附属第一医院血液内科,广州510080 [2]中山大学附属第一医院东院全科医学科,广州510080

出　　处：《医学研究杂志》2021年第9期107-110,共4页Journal of Medical Research

基　　金：广东省自然科学基金资助项目(18zxxt23)。

摘　　要：目的探讨在多发性骨髓瘤(multiple myeloma,MM)细胞中乙酰肝素酶(heparanase,HPSE)与Runt相关的转录因子2(runt related transcription factor 2,Runx2)之间的关系。方法采用Western blot法比较不同HPSE表达水平的骨髓瘤细胞中转录因子Runx2的表达含量,采用染色质免疫共沉淀的方法比较不同HPSE表达水平的骨髓瘤细胞中转录因子Runx2结合RANKL、MMP9启动子区域的DNA含量。结果在骨髓瘤细胞中转录因子Runx2的表达含量同步于HPSE表达水平,HPSE高表达的骨髓瘤细胞中转录因子Runx2结合RANKL、MMP9启动子区域的DNA含量增加。结论HPSE通过转录因子Runx2调控骨髓瘤细胞功能,HPSE及Runx2可能是潜在的治疗MM的分子靶点。Objective To investigate the relationship between heparanase(HPSE)and Runt related transcription factor 2(Runx2)in multiple myeloma(MM)cells.Methods Western blot was used to compare the expression of transcription factor Runx2 in myeloma cells with different HPSE expression levels,and chromatin immunoprecipitation was used to compare the DNA content of transcription factor Runx2 combined with RANKL and MMP9 promoter regions in myeloma cells with different HPSE expression levels.Results In our study,we found that the expression level of transcription factor Runx2 in myeloma cells was synchronized with the expression level of HPSE.In myeloma cells with high expression of HPSE,and the DNA content of transcription factor Runx2 binding to RANKL and MMP9 promoter regions was increased.Conclusion HPSE regulates myeloma cell function through the transcription factor Runx2.HPSE and Runx2 may be potential molecular targets for the treatment of MM.

关 键 词：乙酰肝素酶 RUNX2 多发性骨髓瘤 

分 类 号：R733.3[医药卫生—肿瘤]