Runjing extract promotes spermatogenesis in rats with ornidazole-induced oligoasthenoteratozoospermia through extracellular signal-regulated kinase signalling,and regulating vimentin expression  

作　　者：YANG Kai LI Shugen ZHANG Tianyu DONG Panpan ZENG Qingqi 

机构地区：[1]Department of Andrology,Zhangjiagang TCM Hospital Affiliated to Nanjing University of Chinese Medicine,Zhangjiagang 215600,China [2]Department of Urology,Suzhou Municipal Hospital,Suzhou 215002,China [3]Department of Andrology,The Affiliated Hospital of Nanjing University of Chinese Medicine,Nanjing 210029,China [4]Department of Andrology,Tongde Hospital of Zhejiang Province,Hangzhou 310012,China

出　　处：《Journal of Traditional Chinese Medicine》2021年第4期581-587,共7页中医杂志（英文版）

基　　金：Supported by the Suzhou Science and Technology Development Plan:Based on the Theory of Collaterals Disease and Damp Disease Explore Promoting Dampness and Dredging Collateral Methods through P38mapk Signaling Pathways Regulate Inflammation Factor of ammatory MechanismⅢRe Type Prostatitis Anti-inflsearch(No.Sysd2018174);Zhangjiagang Science and Technology Plan:Based on the Mapk Pathway Research Runjing Decoction to Oxidative Stress Injury Caused by Weak SD Rats and the Protective Effects of Disease Mechanism Research,(No.ZKS1835);Youth Science and Technology Project of Zhangjiagang Health Plan:Based on the Theory of Essence Chamber to Explore Runjing Decoction Treating Idiopathic Less Weak Sperm Disease Clinical Research and the Analysis of Security(No.ZJGQNKJ201927)。

摘　　要：OBJECTIVE:To investigate the efficacy of Runjing(RJ)extract on oligoasthenoteratozoospermia(OAT)induced by ornidazole(ORN)in rats,and to study the underlying mechanism.METHODS:Twenty-four adult male Sprague-Dawley rats were treated with normal saline(control),ORN(OAT model),ORN+4.725 g-dose)and ORN+18.9 g·kg^(-1)·d^(-1) RJ extract(low·kg^(-1)·d^(-1) RJ extract(high-dose)for 4 weeks.The rats were then euthanized and sperm and testis samples were collected for analysis.Sperm count,motility and morphology were calculated by sperm suspension from cauda epididymis.Testicular histopathological changes were analyzed by hematoxylin and eosin staining and Td T mediated d UTP nick end labelling.Moreover,the expression of vimentin and extracellular signal-regulated kinase(ERK)were examined through Western blot,and the distribution of vimentin was detected via immunohistochemistry.RESULTS:ORN successfully induces seminiferous epithelium injury,cellular apoptosis,and finally OAT(P<0.05).However,both low-dose and highdose RJ extract partially rescues the altered phenotypes(P<0.05).Moreover,the expressions of vimentin and ERK were significantly altered in ORN testes(all P<0.001),while RJ extract partially reversed these effects(P<0.01 or P<0.001).CONCLUSION:RJ extract can help maintain spermatogenesis through ERK signalling,and regulating vimentin expression.

关 键 词：SPERMATOGENESIS OLIGOSPERMIA VIMENTIN extracellular signal-regulated MAP kinases Runjing 

分 类 号：R285[医药卫生—中药学]