HOXC4 up-regulates NF-κB signaling and promotes the cell proliferation to drive development of human hematopoiesis, especially CD43+ cells  

作　　者：Jiahui Zeng Wencui Sun Jing Chang Danying Yia Lijiao Zhu Yonggang Zhang Xu Pan Ya Zhou Mowen Lai Guohui Bian Qiongxiu Zhou Jiaxin Liu Bo Chen FengMa 

机构地区：[1]Institute of Blood Transfusion,Chinese Academy of Medical Sciences&Peking Union Medical College(CAMS&PUMC),Chengdu 610052,China [2]State Key Laboratory of Biotherapy,Sichuan University,Chengdu 61006,China [3]State Key Laboratory of Experimental Hematology,CAMS&PUMC,Tianjin 300020,China

出　　处：《Blood Science》2020年第4期117-128,共12页血液科学（英文）

基　　金：This work was supported by awards from the CAMS Initiatives for Innovative Medicine(2016-I2M-1-018 to F.Ma and 2017-I2M-3-021 to J.X.Liu);Sichuan Provincial Science and Technology Department Key R&D projects(020YFSY0023 to B.Chen);the Chengdu Science and Technology Project-Technology Innovation R&D(2018-YF05-01341-SN to B.Chen).

摘　　要：The hematopoietic function of HOXC4 has not been extensively investigated.Our research indicated that induction of HOXC4 in co-culture system from D10 significantly promoted productions of most hematopoietic progenitor cells.CD34−CD43+cells could be clearly classified into CD34−CD43^(low) and CD34−CD43^(high) sub-populations at D14.The former cells had greater myelogenic potential,and their production was not significantly influenced by induction of HOXC4.By contrast,the latter cells had greater potential to differentiate into megakaryocytes and erythroid cells,and thus had properties of erythroid–megakaryocyte common progenitors,which abundance was increased by∼2-fold when HOXC4 was induced from D10.For CD34−CD43^(low),CD34+CD43+,and CD34−CD43^(high) sub-populations,CD43 level served as a natural index for the tendency to undergo hematopoiesis.Induction of HOXC4 from D10 caused more CD43+cells sustain in S-phase with up-regulation of NF-κB signaling,which could be counteracted by inhibition of NF-κB signaling.These observations suggested that promotion of hematopoiesis by HOXC4 is closely related to NF-κB signaling and a change in cell-cycle status,which containing potential of clinical applications.

关 键 词：CD43 Erythroid–megakaryocyte common progenitor(EMkP) HEMATOPOIESIS HOXC4 Human embryonic stem cells(hESCs) Inducible expression 

分 类 号：R73[医药卫生—肿瘤]