Zn^(2+)抑制香菇吸附重金属镉工艺优化研究  

Optimization of Zn Inhibited Adsorption Process on Cadmium Adsorption of Lentinus Edodes

作　　者：乔鑫 李昭敏 裴亚琼 李蕙蕙[1] 汪师帅[1] 张亮子 边银丙[2] QIAO Xin;LI Zhaomin;PEI Yaqiong;LI Huihui;WANG Shishuai;ZHANG Liangzi;BIAN Yinbing(College of Food Technology,Wuhan Business University,Wuhan 430056,China;Institute of Applied Mycology,Huazhong Agricultural University,Wuhan 430071,China)

机构地区：[1]武汉商学院食品科技学院,湖北武汉430056 [2]华中农业大学应用真菌研究所,湖北武汉430071

出　　处：《食品科技》2021年第8期71-75,共5页Food Science and Technology

基　　金：湖北省教育厅科学技术研究计划指导性项目(B2020235)。

摘　　要：为解决香菇重金属镉超标,通过向香菇培养基添加Zn^(2+)的方法来降低镉含量,研究在不同pH值、不同温度以及不同Zn^(2+)添加浓度下香菇菌丝吸附重金属镉的影响,并且运用线性回归方程优化最低吸附镉工艺,同时应用透射电镜观察Zn^(2+)添加后菌丝中镉分布的变化。结果表明,弱酸(pH=4~7)下Zn^(2+)(10 mg/mL)更有利于阻碍菌丝吸收镉,高温(30 ℃)下Zn^(2+)对抑制香菇菌丝吸附镉更有效,Zn^(2+)浓度越接近20 mg/mL香菇菌丝吸附镉含量越低。经回归分析,pH值为5、温度为25 ℃、Zn^(2+)浓度为18.5 μg/mL时,香菇菌丝吸附重金属镉的含量最低,为19.8 μg/g。透射电镜观察到Zn^(2+)减轻了重金属镉对菌丝形态结构的毒害,菌丝内部锌和镉含量高于菌丝外部,且锌含量始终高于镉含量。To solve excessive heavy metals cadmium of Lentinus edodes,this article describes the method of reducing effectively cadmium content by adding Zn^(2+) to culture medium.The change on cadmium adsorption of L.edodes mycelia under different pH,temperature and concentration of Zn^(2+) were studied,using the linear regression equation to optimize the lowest cadmium adsorption process,and observing cadmium distribution change by TEM (Transmission electron microscope).The results showed that Zn^(2+) was more beneficial to reduce the cadmium adsorption of L.edodes mycelia under weak acid (pH=4~7),the stronger the inhibition ability of Zn^(2+) on L.edodes mycelia was under high temperature (30 ℃),and the closer that this value is to 20 mg/L,the cadmium adsorption content of L.edodes mycelia could be more effectively reduced.Based on single factor results and the regression equation calculates,when pH is 5,temperature is 25 ℃,and Zn^(2+) concentration is 18.5 μg/mL,the content of cadmium adsorption by L.edodes mycelia is the lowest,which is 19.8 μg/g.TEM showed that Zn^(2+) alleviated the toxic effect of morphology and structure from heavy metal Cd.The contents of Zn and Cd inside the mycelium were higher than those outside the mycelium,and the content of Zn was always higher than that of Cd.

关 键 词：镉 锌 香菇 抑制吸附 

分 类 号：TS219[轻工技术与工程—粮食、油脂及植物蛋白工程]