姜黄素超分子包合物对乙醇诱导LO2细胞损伤的保护作用  

Protective Effect of Curcumin/Cyclodextrin Polymer Inclusion Complex on LO2 Cells Damaged by Ethanol

作　　者：范土贵 陈建平 高加龙 钟赛意 秦小明 FAN Tugui;CHEN Jianping;GAO Jialong;ZHONG Saiyi;QIN Xiaoming(College of Food Science and Technology,Guangdong Ocean University,Guangdong Provincial Key Laboratory of Aquatic Product Processing and Safety,Guangdong Provincial Engineering Technology Research Center of Seafood,Guangdong Province Engineering Laboratory for Marine Biological Products,Key Laboratory of Advanced Processing of Aquatic Product of Guangdong Higher Education Institution,Guangdong Provincial Modern Agricultural Science and Technology Innovation Center for Subtropical Fruit and Vegetable Processing,Zhanjiang 524088,China;Dalian Polytechnic University,Collaborative Innovation Center of Seafood Deep Processing,Dalian 116034,China)

机构地区：[1]广东海洋大学食品科技学院,广东省水产品加工与安全重点实验室,广东省海洋食品工程技术研究中心,广东省海洋生物制品工程实验室,水产品深加工广东普通高等学校重点实验室,广东省亚热带果蔬加工科技创新中心,广东湛江524088 [2]大连工业大学,海洋食品精深加工关键技术省部共建协同创新中心,辽宁大连116034

出　　处：《食品工业科技》2021年第18期366-371,共6页Science and Technology of Food Industry

基　　金：广东省自然科学基金面上项目(2020A1515010860,2021A1515012455);广东海洋大学创新强校项目(230419100);广东海洋大学“南海学者计划”项目(002029002009)。

摘　　要：本文考察了姜黄素超分子包合物(Curcumin/Cyclodextrin polymer inclusion complex,CUR/CDP)对乙醇诱导LO2细胞损伤的保护作用。采用MTT法建立乙醇诱导LO2细胞损伤的模型,通过谷氨酸氨基转移酶(ALT)、天门冬氨酸氨基转移酶(AST)、乳酸脱氢酶(LDH)、超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSHPX)、丙二醛(MDA)和活性氧(ROS)等试剂盒考察了CUR/CDP对乙醇诱导LO2细胞损伤的保护效果。结果表明,经CUR/CDP(80μg/mL)处理后,LO2细胞的存活率从54.75%±8.97%(模型对照组)提高到85.27%±2.64%,且细胞培养液中ALT、AST和LDH活力分别从(26.47±0.90)、(41.02±4.41)、(63.77±4.95)U/L(模型对照组)降低到(16.17±0.42)、(22.62±0.79)、(32.25±1.69)U/L(P<0.01),LO2细胞内GSH-PX、SOD活力分别从(21.82±1.34)、(8.45±1.11)U/mg prot(模型对照组)升高到(36.70±0.56)、(16.47±1.27)U/mg prot,LO2细胞内MDA和ROS含量分别从(1.19±0.15)nmol/mg prot、(198.02%±11.76%)(模型对照组)降低到(0.72±0.05)nmol/mg prot、(110.87%±10.22%)(P<0.01)。综上所述,CUR/CDP通过提高LO2细胞相关抗氧化酶的活力和降低细胞内ROS含量来改善乙醇诱导LO2细胞的损伤。This study investigated the protective effect of curcumin/cyclodextrin polymer inclusion complex(CUR/CDP)on LO2 cells damaged by ethanol.The MTT method was used to establish a model of LO2 cells damaged by ethanol.Glutamate alanine aminotransferase(ALT),aspartate aminotransferase(AST),lactate dehydrogenase(LDH),superoxide dismutase(SOD),glutathione peroxidase(GSH-PX),malondialdehyde(MDA)and reactive oxygen species(ROS)kits were used to investigate the protective effect of CUR/CDP on LO2 cells damaged by ethanol.The results showed that after CUR/CDP(80μg/mL)treatment,the cell activity of LO2 cells increased from(54.75%±8.97%)(model group)to 85.27%±2.64%,and the activities of ALT,AST,and LDH in the LO2 cell culture medium decreased from(26.47±0.90),(41.02±4.41),(63.77±4.95)U/L(model group)to(16.17±0.42),(22.62±0.79),(32.25±1.69)U/L(P<0.01),respectively;the activities of GSH-PX and SOD in LO2 cells increased from(21.82±1.34),(8.45±1.11)U/mg prot(model group)to(36.70±0.56),(16.47±1.27)U/mg prot;the content of MDA and ROS in LO2 cells decreased from(1.19±0.15)nmol/mg prot,(198.02%±11.76%)(model group)to(0.72±0.05)nmol/mg prot,(110.87%±10.22%)(P<0.01),respectively.In conclusion,CUR/CDP could improve the damage of LO2 cells induced by ethanol by increasing the activity of antioxidant enzymes and reducing the content of ROS.

关 键 词：姜黄素超分子包合物 LO2 细胞 乙醇损伤 保护作用 活性氧 

分 类 号：TS201.2[轻工技术与工程—食品科学]