簕菜提取物对酒精性肝损伤大鼠的保护作用  

Protective Effect of Acanthopanax trifoliatus Extract on Rats with Alcoholic Liver Injury

作　　者：李冬梅[1] 李志阳 杨君[1] 郝志明 杨洁[1] 林春华 LI Dongmei;LI Zhiyang;YANG Jun;HAO Zhiming;YANG Jie;LIN Chunhua(Institute of Tropical Agriculture and Forestry,Guangdong AIB Polytechnic College,Guangzhou 510503,China)

机构地区：[1]广东农工商职业技术学院热带农林学院,广东广州510503

出　　处：《食品工业科技》2021年第18期380-386,共7页Science and Technology of Food Industry

基　　金：广州市科技局科研项目(201904010283);广东省南亚热带休闲农业工程技术研究中心资助项目(粤科函产学研字[2017]1649号)。

摘　　要：目的:探讨簕菜不同提取物的解酒护肝作用,为簕菜解酒护肝功能食品的研发提供依据。方法:建立白酒灌胃大鼠致酒精肝损伤模型,考察簕菜醇提物和水提物对大鼠酒精耐受、睡眠和醒酒时间的影响,检测大鼠血液中乙醇质量浓度、血清中谷丙转氨酶(ALT)、谷草转氨酶(AST)活性,以及大鼠肝脏组织中乙醇脱氢酶(ADH)、乙醛脱氢酶(ALDH)、超氧化物歧化酶(SOD)活性及谷胱甘肽(GSH)、丙二醛(MDA)含量,切片镜检肝细胞形态变化。结果:与酒精模型组比较,除水提物低剂量组对醉酒时间无显著变化(P>0.05)外,簕菜提取物各剂量组均可显著延长酒精耐受时间,并缩短睡眠、醒酒时间(P<0.05,P<0.01);簕菜提取物均可极显著降低血清中乙醇含量(P<0.01);除水提低剂量组AST活力无显著变化外(P>0.05),簕菜提取物各剂量组均能显著抑制酒精引起的大鼠血清ALT和AST活性升高(P<0.05);极显著增加肝脏组织中ADH、ALDH、SOD活力和GSH含量(P<0.01),降低MDA含量(P<0.01);肝细胞镜检结果显示,簕菜提取物均能在一定程度上减轻酒精导致的肝细胞变性程度。结论:簕菜醇提物、水提物均具有较好的防醉酒和解酒护肝作用,比较而言,醇提物效果优于水提物。Objective:To explore the hepatoprotective effects of different extracts of Acanthopanax trifoliatu,and provide a basis for the research and development of Acanthopanax trifoliatus anti-alcohol and liver-protecting functional foods.Method:Rats were fed by liquor(56%ethanol)to induce acute liver injury models,to study the effects of alcohol extract and water extract of Acanthopanax trifoliatus on the alcohol tolerance,sleep and sober time of rats.The concentration of ethanol in blood and the activites of ALT&AST in serum,activites of ADH,ALDH,SOD and contents of GSH and MDA in rat liver tissue were measured,and the changes of liver cell morphology by slice microscopic examination was observed.Results:Compared with the alcohol model group,except for the low-dose water extract group,there was no significant change in the drunken time(P>0.05),the various doses extract significantly prolonged the alcohol tolerance time and shortened the sleep and sober time(P<0.05,P<0.01);the various doses extract significantly reduced the ethanol mass concentration(P<0.01);except for the low-dose water extract group,there was no significant change in AST activity(P>0.05),all dose groups of extract could significantly inhibit the increase in rat serum ALT and AST activities caused by alcohol(P<0.05);all extracts significantly increased the activity of ADH,ALDH,SOD and GSH content in liver tissues(P<0.01),and reduced the content of MDA in liver tissues(P<0.01).The results of hepatocyte microscopy showed that all extracts could reduce the degree of liver cell degeneration caused by alcohol to a certain extent.Conclusion:Both the alcoholic and water extracts had good anti-alcoholic and liver-protective effects,in comparison,the alcohol extract was slightly better than the water extract.

关 键 词：簕菜 醇提物 水提物 酒精性肝损伤 大鼠 解酒护肝 

分 类 号：TS201.4[轻工技术与工程—食品科学]