苦丁皂苷D的分离纯化及其纳米粒的制备表征  

Separation and Purification of Kudinoside D and Its Nanoparticle Preparation and Characterization

作　　者：胡夏恬 张凤清[1] 于敏[2] HU Xiatian;ZHANG Fengqing;YU Min(College of Chemistry and Life Sciences,Changchun University of Technology,Changchun 130012,China;Jinlin Provincial Academy of Traditional Chinese Medicine,Changchun 130118,China)

机构地区：[1]长春工业大学化学与生命科学学院,吉林长春130012 [2]吉林省中医药科学院,吉林长春130118

出　　处：《食品工业科技》2021年第19期15-20,共6页Science and Technology of Food Industry

基　　金：吉林省发展和改革委员会(2020C032-6);吉林省卫生计生科研计划项目(2014z206);吉林省中医药科技项目(2014-ZD28)。

摘　　要：目的:为了提高苦丁皂苷D的水溶性。方法:采用常压柱层析法、半制备高效液相色谱法等手段从苦丁茶冬青中分离纯化皂苷,获得化合物苦丁皂苷D(Kudinoside D)。以聚谷氨酸(γ-PGA)和L-苯丙氨酸乙酯(LPAE)为原料,分别采用沉淀法、透析法制备苦丁皂苷D纳米粒,利用透射电镜和动态纳米粒度仪对纳米粒理化性质进行表征,利用透析法考察其体外释药特性。结果:与沉淀法相比,透析法制备的苦丁皂苷D纳米粒的包封率和载药量分别提高了1.5、4.5倍,包封率达65.46%,载药量达13.24%。苦丁皂苷D纳米粒呈规则球形,平均粒径为(75±25)nm,Zeta电势为33.7。结论:体外释药实验表明苦丁皂苷D纳米粒可提高药物的水溶性,具有良好的体外缓释效果。Objective:To improve the water solubility of Kudinoside D.Methods:Using atmospheric column chromatography,semi-preparative high performance liquid chromatography and other means to separate and purify saponins from Ilex kudingcha C.J.Tseng,the compound Kudinoside D was obtained.Using polyglutamic acid(γ-PGA)and L-phenylalanine ethyl ester(L-PAE)as raw materials,Kudinoside D nanoparticles were prepared by precipitation method and dialysis method respectively.The physical and chemical properties of nanoparticles were characterized by transmission electron microscopy and dynamic nanoparticle size analyzer,and their in vitro drug release characteristics were investigated by dialysis.Results:Compared with the precipitation method,the encapsulation efficiency and drug loading of Kudinoside D nanoparticles prepared by the dialysis method were increased by 1.5 and 4.5 times,respectively,the encapsulation rate reached 65.46%,and the drug loading reached 13.24%.Kudinoside D nanoparticles had a regular spherical shape with an average particle size of(75±25)nm and a Zeta potential of 33.7.Conclusion:In vitro drug release experiments show that Kudinoside D nanoparticles can significantly improve the water solubility of the drug and have a good sustained release effect in vitro.

关 键 词：苦丁皂苷D γ-PGA-PAE 纳米粒 表征 分离纯化 

分 类 号：TS101.8[轻工技术与工程—纺织工程]