Visualization of the Oncolytic Alphavirus M1 Life Cycle in Cancer Cells  

作　　者：Jia Dan Lin Nie Xudong Jia Cuiying Xu Jing Cai Yuan Lin Jun Hu Wenbo Zhu Yinyin Li Dong Chen Ying Liu Cheng Hu Guangmei Yan Jiankai Liang Qinfen Zhang 

机构地区：[1]Department of Pharmacology,Zhongshan School of Medicine,Sun Yat-Sen University,Guangzhou 510080,China [2]State Key Lab for Biocontrol,School of Life Sciences,Sun Yat-Sen University,Guangzhou 510275,China [3]Department of Infectious Diseases,The Third Affiliated Hospital of Sun Yat-Sen University,Guangzhou 510630,China [4]Department of Urology,The Third Affiliated Hospital of Sun Yat-Sen University,Guangzhou 510630,China

出　　处：《Virologica Sinica》2021年第4期655-666,共12页中国病毒学（英文版）

基　　金：This research was supported by the Science and Technology Program of Guangzhou(201707020003);the Pioneering Talents Project of Guangzhou Development Zone,Guangdong Province(CY2018-012);the Guangzhou People's Livelihood Science and Technology Tackling Key Project(201803010113);the Young Teacher Training Program of Sun Yat-sen University(20ykpy22);the National Natural Science Foundation of China(No.81802536).

摘　　要：Oncolytic alphavirus M1 has been shown to selectively target and kill cancer cells,but cytopathic morphologies induced by M1 virus and the life cycle of the M1 strain in cancer cells remain unclear.Here,we study the key stages of M1 virus infection and replication in the M1 virus-sensitive HepG2 liver cancer cell line by transmission electron microscopy,specifically examining viral entry,assembly,maturation and release.We found that M1 virus induces vacuolization of cancer cells during infection and ultimately nuclear marginalization,a typical indicator of apoptosis.Specifically,our results suggest that the endoplasmic reticulum participates in the assembly of nucleocapsids.In the early and late stage of infection,three kinds of special cytopathic vacuoles are formed and appear to be involved in the replication,maturation and release of the virus.Taken together,our data displayed the process of M1 virus infection of tumor cells and provide the structural basis for the study of M1 virus-host interactions.

关 键 词：Oncolytic virus M1 ALPHAVIRUS Electron microscopy Life cycle of virus 

分 类 号：R36[医药卫生—病理学]